B1911
BCIP®/NBT
alkaline phosphatase substrate, chromogenic, liquid
Synonyma:
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution
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O této položce
UNSPSC Code:
12352204
NACRES:
NA.83
Technický servis
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BCIP®/NBT Liquid Substrate System, ready to use solution
Quality Segment
form
liquid
technique(s)
protein detection: suitable
storage temp.
2-8°C
Application
A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:
- for staining alkaline phosphatase(ALP) in bone marrow stromal cells
- as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
- as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.
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Skladovací třída
12 - Non Combustible Liquids
Co se děje?
WGK 1
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
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