B9285

5-Bromo-2′-deoxyuridine

≥99%, synthetic (organic), powder

• Synonyma: 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
• Empirický vzorec (Hillův zápis): C₉H₁₁BrN₂O₅
• Číslo CAS: 59-14-3
• Molekulová hmotnost: 307.10
• NACRES: NA.51
• PubChem Substance ID: 24892132
• UNSPSC Code: 41106305
• EC Number: 200-415-9
• MDL number: MFCD00006529
• Beilstein/REAXYS Number: 30395
• eCl@ss: 32119108
• Assay: ≥99%
• Biological source: synthetic (organic)
• Form: powder
• Solubility: NH₄OH: 0.1 M at 20 °C, clear, colorless
• Storage temp.: −20°C

Vlastnosti

• Název produktu: 5-Bromo-2′-deoxyuridine, BioUltra, ≥99%
• biological source: synthetic (organic)
• Quality Segment: 200
• product line: BioUltra
• assay: ≥99%
• form: powder
• impurities: ≤0.005% Phosphorus (P), ≤0.1% Insoluble matter
• ign. residue: ≤0.1%
• mp: 191-194 °C (dec.) (lit.)
• solubility: NH₄OH: 0.1 M at 20 °C, clear, colorless
• cation traces: Al: ≤0.0005%, Ca: ≤0.01%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH₄⁺: ≤0.05%, Na: ≤0.05%, Pb: ≤0.001%, Zn: ≤0.0005%
• storage temp.: −20°C
• SMILES string: OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• InChI key: WOVKYSAHUYNSMH-RRKCRQDMSA-N

Popis

General description

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light.

Application

5-Bromo-2′-deoxyuridine has been used:

• to label proliferating cells in pregnant mice in cell proliferation assay
• to study the time course of cell proliferation at various times following ischemia, to confirm re-entry of MKI67+ cells in cell cycle to germ cells
• in 5′-Bromo-2′Deoxyuridine (BrdUrd) staining of fibroblasts

Biochem/physiol Actions

5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Bezpečnostní informace

• Piktogramy: GHS08
• Výstražné slovo: Danger
• Kódy nebezpečí: H340,H361fd
• Bezpečnostní pokyny: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 1B - Repr. 2
• Skladovací třída: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Co se děje?: WGK 2
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)