F3385

Fetuin from fetal bovine serum

lyophilized powder, BioReagent, suitable for cell culture

• Synonyma: Fetuin from fetal calf serum
• Číslo CAS: 9014-81-7
• UNSPSC Code: 12352202
• EC Number: 232-762-7
• NACRES: NA.75
• MDL number: MFCD00131055
• Form: lyophilized powder
• Mol wt: 48.4 kDa

Vlastnosti

• product line: BioReagent
• Quality Segment: 200
• form: lyophilized powder
• mol wt: 48.4 kDa
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.3% free N-acetylneuraminic acid
• solubility: H₂O: 1 mg/mL
• storage temp.: 2-8°C

Popis

General description

Fetuin from fetal bovine serum is a plasma glycoprotein. It is a 64 kDa glycoprotein, which is produced by the liver and adipose tissue.
A glycoprotein derived from FBS that is used as Fetuin has been used at a concentration of 500 mg/ml to supplement serum free F12 medium (along with insulin, transferrin and 2-mercaptoethanol) in culture of embryonal carcinoma cells.

Application

Fetuin from fetal bovine serum has been used:

• as a substrate to determine neuraminidase activity
• to validate the specificity of sialidases
• to stabilize m-calpain

Biochem/physiol Actions

Fetuin acts as an inhibitor of insulin receptor tyrosine kinase. It modulates bone remodeling and calcium metabolism. Fetuin regulates vascular calcification, insulin resistance, protease activity control, keratinocytes migration and breast tumor cell proliferative signaling. It functions as a biomarker for neurodegenerative diseases. Fetuin exhibits anti-inflammatory property.

Preparation Note

Prepared by ammonium sulfate fractionation of fetal bovine serum by the method of Pederson, K.O., J. Phys. and Colloid Chem., 51, 164 (1947).

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• Co se děje?: WGK 3
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)