M5655

Thiazolyl Blue Tetrazolium Bromide

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

• Synonyma: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
• Empirický vzorec (Hillův zápis): C₁₈H₁₆BrN₅S
• Číslo CAS: 298-93-1
• Molekulová hmotnost: 414.32
• UNSPSC Code: 12352207
• NACRES: NA.75
• PubChem Substance ID: 24897030
• EC Number: 206-069-5
• Beilstein/REAXYS Number: 4081397
• MDL number: MFCD00011964

Vlastnosti

• product line: BioReagent
• Quality Segment: 200
• assay: ≥97.5% (HPLC)
• form: powder
• technique(s): cell culture | insect: suitable, cell culture | mammalian: suitable
• mp: 195 °C (dec.) (lit.)
• solubility: H₂O: 5 mg/mL
• storage temp.: 2-8°C
• SMILES string: [Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
• InChI: 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• InChI key: AZKSAVLVSZKNRD-UHFFFAOYSA-M

Popis

Application

Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay). It has also been used to determine cell proliferation.

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Bezpečnostní informace

• Piktogramy: GHS08,GHS07
• Výstražné slovo: Warning
• Kódy nebezpečí: H315,H319,H335,H341
• Bezpečnostní pokyny: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• Cílové orgány: Respiratory system
• Skladovací třída: 11 - Combustible Solids
• Co se děje?: WGK 3
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)