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Merck

MAK165

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (red fluorescence)

Synonyma:

Hydrogen Peroxide Quantification Kit

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O této položce

NACRES:
NA.84
UNSPSC Code:
12161503
eCl@ss:
32160703
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usage

sufficient for 500 fluorometric tests (red fluorescence)

detection method

fluorometric

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders

storage temp.

−20°C

General description

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Application

This kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

Biochem/physiol Actions

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (λex = 540/λem = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.

Features and Benefits

Compatible with high-throughput handling systems.


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Piktogramy

Health hazardExclamation mark

Výstražné slovo

Danger

Kódy nebezpečí

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1

Skladovací třída

10 - Combustible liquids

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

WGK

WGK 3



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