N8271

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

recombinant, expressed in E. coli, buffered aqueous solution

• Synonyma: Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase
• Číslo CAS: 9001-67-6
• UNSPSC Code: 12352204
• NACRES: NA.32
• Číslo EC: 3.2.1.18 (BRENDA, IUBMB)
• MDL number: MFCD00131711
• Specific activity: ≥135 units/mg protein
• Recombinant: expressed in E. coli

Vlastnosti

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: buffered aqueous solution
• specific activity: ≥135 units/mg protein
• mol wt: 88 kDa, 95 kDa
• foreign activity: β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Popis

Biochem/physiol Actions

Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.

Preparation Note

Expressed in glycosidase-free hosts.

Other Notes

One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• Co se děje?: WGK 1
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)