The control primers specific for the mouse GAPDH promoter are as follows:
FOR: 5'-CCTCTGCGCCCTTGAGCTAGGA-3’
REV: 5'-CACAAGAAGATGCGGCCGTCTC-3’
RIP
Imprint® RNA Immunoprecipitation Kit
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
Synonyma:
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Velikost balení | Skladová položka | Dostupnost | Cena |
|---|---|---|---|
| 12 reactions | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 18 100,00 Kč |
O této položce
NACRES:
NA.54
UNSPSC Code:
41116158
18 100,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohliQuality Level
General description
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.
Learn more about this product and view application data.
Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
Application
Imprint® RNA Immunoprecipitation Kit has been used for:
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits
- Xtra Protein A magnetic beads with higher binding capacity
- Step-by-step protocols for cell lysis and immunoprecipitation
- RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
- Negative control and antibody included
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Tato položka | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Součásti soupravy, které jsou dostupné také samostatně
Č. produktu
Popis
Bezpečností list & Stanovení ceny
- IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
- Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Skladovací třída
3 - Flammable liquids
wgk
WGK 3
flash_point_f
100.4 °F
flash_point_c
38 °C
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
Vyberte jednu z posledních verzí:
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Protokoly
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
Sortimentní položky
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Související obsah
Instructions
Qiyuan Wang et al.
Journal of cellular biochemistry, 118(11), 3775-3784 (2017-04-06)
Osteoarthritis (OA) is characterized by progressive destruction of articular cartilage, resulting in significant disability. Inflammatory cytokines commonly initiate the extreme changes in the synovium and cartilage microenvironment of the OA patients, subsequently resulting in cell dysfunctions, especially synoviocyte dysfunction. We
Yiwei Liao et al.
Journal of cellular biochemistry, 118(7), 1889-1899 (2017-01-26)
Temozolomide (TMZ)-based chemotherapy is a standard strategy for glioma, while chemoresistance remains a major therapeutic challenge. Recent evidence highlights the crucial regulatory roles of long non-coding RNAs (lncRNA) in tumor biology. However, the roles and regulatory mechanisms of lncRNA cancer
Shuai Xiao et al.
DNA and cell biology, 37(2), 117-125 (2018-02-08)
P73 antisense RNA 1T (non-protein coding), known as TP73-AS1 or PDAM, is a long noncoding RNA (lncRNA), which may regulate apoptosis by regulation of p53-dependent antiapoptotic genes. An abnormal change of TP73-AS1 expression was noticed in cancers. The effects of
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| 03-902 | 04065265138673 |
| 03-901 | 04065265138680 |
| SAB1400734-50UG | 04061831492439 |
| 03-110 | 04053252679209 |
| 03-176 | 04053252291005 |
| 03-111 | 04053252679216 |
| 03-191 | 04053252518928 |
| 03-119 | 04053252376474 |
| 03-204 | 04053252682308 |
| 03-102 | 04053252583209 |
| 03-101 | 04053252320415 |
| 03-178 | 04053252480614 |
| 03-104 | 04053252468421 |
| 03-200 | 04053252480621 |
| 03-179 | 04053252376481 |
| 03-196 | 04053252376498 |
| SAB5701280-100UL | 04065267666549 |
| 17-704 | 04053252025426 |
| 03-248 | 04053252756535 |
| 03-113 | 04053252679759 |
| 03-107 | 04053252679186 |
| 03-206 | 04053252556128 |
| 03-900 | 04065265138697 |
| 03-181 | 04053252682292 |
| SAB5701958-100UL | 04065267679358 |
-
Please suggest the appropriate control primer set recommended for use with mouse tissue samples while using EZ Magna Chip A/G kit, 17-10086. It includes control antibodies (anti-RNA pol II) and a control primer set intended for the human GAPDH promoter.
1 answer-
Helpful?
-