T5398

Transglutaminase from guinea pig liver

lyophilized powder, ≥1.5 units/mg protein

• Synonyma: Protein-Glutamine-γ-Glutamyltransferase, Protein-glutamine:amine γ-glutamyltransferase
• Číslo CAS: 80146-85-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• Číslo EC: 2.3.2.13 (BRENDA, IUBMB)
• MDL number: MFCD00132446
• Specific activity: ≥1.5 units/mg protein

Vlastnosti

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥1.5 units/mg protein
• mol wt: 76.6 kDa
• composition: Protein, ≥80% modified Warburg-Christian
• solubility: H₂O: soluble 1.0 mg/mL, clear
• application(s): diagnostic assay manufacturing
• shipped in: dry ice
• storage temp.: −20°C

Popis

Application

Transglutaminase has been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington′s disease and Alzheimer′s disease.Transglutaminase has also been used in a study to develop a nonradioactive dot blot assay for transglutaminase activity.

This product from Sigma has been used to demonstrate that tissue transglutaminase (tTG) selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity. It has also been used to assess immune responses to A-gliadin peptides. Furthermore, it has been used to demonstrate that tTG selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease.

Biochem/physiol Actions

Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa. It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium. It catalyzes the incorporation of small molecular weight amines into γ-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross linking of proteins that results in the formation of γ-glutamyl-ε-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme.

Physical form

Lyophilized powder containing Tris and dithioerythritol

Other Notes

One unit will catalyze the formation of 1.0 μmole of hydroxamate per min from Nα-Z-Gln-Gly and hydroxylamine at pH 6.0 at 37 °C. (L-Glutamic acid γ-monohydroxamate is the standard.)

Bezpečnostní informace

• Piktogramy: GHS08
• Výstražné slovo: Danger
• Kódy nebezpečí: H334
• Bezpečnostní pokyny: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Skladovací třída: 11 - Combustible Solids
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)