TDF100A

Total Dietary Fiber Assay Kit

sufficient for ~100 assays

• NACRES: NA.84
• UNSPSC Code: 12164500

Vlastnosti

• usage: sufficient for ~100 assays
• Quality Segment: 100
• technique(s): photometry: suitable
• application(s): food and beverages; general analytical
• storage temp.: 2-8°C

Popis

Application

This assay determines the total dietary fiber content of food using a combination of enzymatic and gravimetric methods. Samples of dried, fat-free foods are gelatinized with heat stable α-amylase and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample. Ethanol is added to precipitate the soluble dietary fiber. The residue is then filtered and washed with ethanol and acetone. After drying, the residue is weighed. Half of the samples are analyzed for protein and the others are ashed. Total dietary fiber is the weight of the residue less the weight of the protein and ash.

Biochem/physiol Actions

Uses a combination of enzymatic and gravimetric methods. This procedure is based on the method published in Official Methods of Analysis, 15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990).

Other Notes

Reagents for the determination of total dietary fiber.

Pouze součásti soupravy

• α-Amylase, Heat-stable 10 mL
• Amyloglucosidase 10 mL
• Celite 50 g
• Protease 500 mg

Bezpečnostní informace

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H315,H318,H334,H335,H372,H410
• pcodes: P260 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
• target_organs: Lungs, Respiratory system
• Skladovací třída: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• flash_point_f: Not applicable
• flash_point_c: Not applicable