U0631

Urea

BioXtra, pH 7.5-9.5 (20 °C, 5 M in H₂O)

• Synonyma: Carbamide, Carbonyldiamide
• Lineární vzorec: NH₂CONH₂
• Číslo CAS: 57-13-6
• Molekulová hmotnost: 60.06
• UNSPSC Code: 12352111
• NACRES: NA.31
• PubChem Substance ID: 24900617
• EC Number: 200-315-5
• Beilstein/REAXYS Number: 635724
• MDL number: MFCD00008022
• eCl@ss: 32150917
• Assay: 98.5-101.5%

Vlastnosti

• product line: BioXtra
• Quality Segment: 200
• assay: 98.5-101.5%
• form: powder, crystals or pellets
• impurities: ≤0.005% Phosphorus (P), ≤0.1% Insoluble matter
• ign. residue: ≤0.1%
• pH: 7.5-9.5 (20 °C, 5 M in H₂O)
• mp: 132-135 °C (lit.)
• solubility: H₂O: 5 M at 20 °C, clear, colorless
• density: 1.335 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤0.05%, sulfate (SO₄^2-): ≤0.05%
• cation traces: Al: ≤0.0005%, Ca: ≤0.001%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH₄⁺: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%
• absorption: ≤0.06 at 260 nm at 5 M, ≤0.06 at 280 nm at 5 M
• application(s): diagnostic assay manufacturing
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759), CA2(760) ; rat ... Ppm1a(24666)

Popis

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Bezpečnostní informace

• Skladovací třída: 13 - Non Combustible Solids
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)