U6504

Urea

suitable for electrophoresis

• Synonyma: Carbamide, Carbonyldiamide
• Lineární vzorec: NH₂CONH₂
• Číslo CAS: 57-13-6
• Molekulová hmotnost: 60.06
• UNSPSC Code: 12352111
• NACRES: NB.22
• PubChem Substance ID: 24900435
• EC Number: 200-315-5
• Beilstein/REAXYS Number: 635724
• MDL number: MFCD00008022
• eCl@ss: 32150917
• Assay: ≥98%
• Technique(s): electrophoresis: suitable, protein electrophoresis: suitable

Vlastnosti

• assay: ≥98%
• Quality Segment: 200
• form: powder or crystals
• technique(s): electrophoresis: suitable, protein electrophoresis: suitable
• impurities: ≤0.005% Ammonia
• mp: 132-135 °C (lit.)
• solubility: H₂O: 100 mg/mL
• density: 1.335 g/mL at 25 °C (lit.)
• cation traces: Fe: ≤5 ppm, Pb: ≤5 ppm
• absorption: ≤0.06 at 260 at 5 M, ≤0.06 at 280 at 5 M
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759), CA2(760) ; rat ... Ppm1a(24666)

Popis

Application

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• WGK: WGK 1
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): Eyeshields, Gloves, type N95 (US)