1.11972

Tryptose sulfite cycloserine agar (base)

GranuCult^® prime, according to ISO 15213, ISO 14189, AOAC^®, APHA, FDA-BAM, granular, for Clostridium spp., pkg of 500 g

• Synonym(s): TSC agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: AOAC^®; APHA; FDA-BAM; ISO 14189; according to ISO 15213
• Quality Level: 200
• sterility: non-sterile
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® prime
• technique(s): microbiological culture: suitable
• pH: 7.4-7.8 (25 °C in H₂O)
• solubility: 42 g/L
• bulk density: 660 kg/m³
• application(s): food and beverages; microbiology
• compatibility: for use with Clostridium perfringens selective supplement (1.00888)
• storage temp.: 15-25°C
• suitability: Clostridium spp.

Description

General description

GranuCult^® Tryptose sulfite cycloserine agar (base) is intended for the isolation and differentiation of Clostridium perfringens. The superior nutrient base provides optimal conditions for the development of Clostridia. Colonies producing hydrogen sulfide are characterized by blackening due to the reaction with sulfite and iron salt. Cycloserine inhibits the accompanying bacterial flora and may cause the colonies that develop to remain smaller. It also reduces diffusion and thus the disturbing blackening around the Clostridium perfringens colonies. TSC agar with MUP contains a fluorogenic substrate for the alkaline and acid phosphatase in addition to cycloserine 4-Methylumbelliferylphosphate (MUP). The acid phosphatase is a high specific indicator for Clostridium perfringens. It splits the fluorogenic substrate MUP, forming 4-methylumbelliferone, which can be identified by its fluorescence in long wave UV light. Thus, a strong suggestion for the presence of Clostridium perfringens can be obtained.

Application

GranuCult^® Tryptose sulfite cycloserine agar (base) is intended for the isolation and differentiation of Clostridium perfringens from food and animal feed, water and other materials. TSC agar with MUP can be prepared using Clostridium perfringens Supplement (1.00888) to detect acid phosphatase by its fluorescence under UV light.

Legal Information

AOAC is a registered trademark of AOAC International

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3