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Merck

12-500

HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer

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100 μG

CZK 5,420.00

CZK 5,420.00

Estimated to ship onFebruary 12, 2026Details

About This Item

UNSPSC Code:
41105330
eCl@ss:
32011807
NACRES:
NA.42

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biological source

human

Quality Level

100

form

liquid

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

shipped in

dry ice

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This Item
RAB0157RAB0152RAB0151
HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer

Sigma-Aldrich

12-500

HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer

Cell-Based ELISA for detecting phospho-EGFR (pTyr992) in cultured cell lines 96 assays

Sigma-Aldrich

RAB0157

Cell-Based ELISA for detecting phospho-EGFR (pTyr992) in cultured cell lines

Cell-Based ELISA for detecting phospho-EGFR (pTyr1068) in cultured cell lines 96 assays

Sigma-Aldrich

RAB0152

Cell-Based ELISA for detecting phospho-EGFR (pTyr1068) in cultured cell lines

Cell-Based ELISA for detecting phospho-EGFR in cultured cell lines, 96 assays

Sigma-Aldrich

RAB0151

Cell-Based ELISA for detecting phospho-EGFR

technique(s)

western blot: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

form

liquid

form

-

form

-

form

-

biological source

human

biological source

-

biological source

-

biological source

-

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

EGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)

Biochem/physiol Actions

HeLa

Physical form

100 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.

Preparation Note

Stable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

EU REACH Annex XIV (Authorisation List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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