14-383

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Name: Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg
Brand: MM
Price: 15600 CZK
Availability: InStock

Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

Synonym(s):

Rho Assay Reagent, Rho Detection Reagent, Rhotekin RBD Assay Reagent

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650 μG

CZK 15,600.00

Estimated to ship onDecember 01, 2025Details

About This Item

UNSPSC Code:

12352200

eCl@ss:

32160405

NACRES:

NA.84

Key Documents

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biological source

mouse

Quality Level

100

manufacturer/tradename

Upstate^®

technique(s)

affinity binding assay: suitable

NCBI accession no.

NM_001015055.1
NM_001015056.1
NM_033046.2

UniProt accession no.

Q9BST9

shipped in

dry ice

Gene Information

mouse ... RTKN(20166)

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This Item	14-455	14-415	14-278
Sigma-Aldrich 14-383 Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg	Sigma-Aldrich 14-455 Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg	Sigma-Aldrich 14-415 Ral Assay Reagent (Ral BP1, agarose)	Sigma-Aldrich 14-278 Ras Assay Reagent (Raf-1 RBD, agarose)
biological source mouse	biological source human	biological source -	biological source human
manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®
technique(s) affinity binding assay: suitable	technique(s) affinity binding assay: suitable	technique(s) affinity binding assay: suitable	technique(s) activity assay: suitable
NCBI accession no. NM_001015055.1	NCBI accession no. NM_018975.2	NCBI accession no. NM_006788.3	NCBI accession no. NM_001085049.1, NM_012219.3
UniProt accession no. Q9BST9	UniProt accession no. Q9NYB0	UniProt accession no. Q15311	UniProt accession no. O14807
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

General description

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.

Product Source: Expressed in E. coli

Physical form

650 µg of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 µg/mL aprotinin, 0.9 µg/mL leupeptin, and 10% glycerol.

Preparation Note

Stable for 6 months at -70°C from date of receipt.

Analysis Note

Routinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778).

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

9002-93-1

EU REACH Annex XIV (Authorisation List)

CAS No.

9002-93-1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cooperativity between the Ras-ERK and Rho-Rho kinase pathways in urokinase-type plasminogen activator-stimulated cell migration.

Minji Jo et al.

The Journal of biological chemistry, 277(14), 12479-12485 (2002-01-24)

Binding of the urokinase-type plasminogen activator (uPA) to its receptor activates diverse cell signaling pathways. How these signals are integrated so that cell physiology is altered remains unclear. In this study, we demonstrated that migration of MCF-7 breast cancer cells

Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA

Porter, K. E., et al

Cardiovascular Research, 61, 745-755 (2004)

Geranylgeranyl diphosphate depletion inhibits breast cancer cell migration.

Amel Dudakovic,Huaxiang Tong,Raymond J Hohl

Investigational New Drugs null

Determination of GTP loading on Rho.

X D Ren et al.

Methods in enzymology, 325, 264-272 (2000-10-19)

Progestogens regulate endothelial actin cytoskeleton and cell movement via the actin-binding protein moesin.

Xiao-Dong Fu, Marina Flamini, Angel Matias Sanchez, Lorenzo Goglia, Maria Silvia Giretti et al.

Molecular Human Reproduction null

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