AB1779SP
Anti-Brain Derived Neurotrophic Factor Antibody
Chemicon®, from rabbit
Synonym(s):
BDNF
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500 μG
CZK 13,300.00
CZK 13,300.00
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A recombinant, preservative-free antibody is available for your target. Try ZRB1136
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BDNF(627)
1 of 4
This Item | AB1779 | AB1513P | AB9042 |
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form serum | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| biological source rabbit | biological source rabbit | biological source sheep | biological source chicken |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| Gene Information human ... BDNF(627) | Gene Information human ... BDNF(627) | Gene Information human ... BDNF(627) | Gene Information human ... BDNF(627) |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
Immunogen
Recombinant Human Brain Derived Neurotrophic Factor (BDNF).
Application
Anti-Brain Derived Neurotrophic Factor Antibody detects level of Brain Derived Neurotrophic Factor & has been published & validated for use in ELISA, WB, IH.
Immunohistochemistry: 5-10μg/mL (see suggested protocol).
Immunoblotting: 5-10μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C. BDNF migrates as 18kDa, with a homodimer at ~27-30kDa. 1:1,000
ELISA: 5-10μg/mL
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Optimal working dilutions must be determined by end user.
Immunoblotting: 5-10μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C. BDNF migrates as 18kDa, with a homodimer at ~27-30kDa. 1:1,000
ELISA: 5-10μg/mL
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Biochem/physiol Actions
Brain Derived Neurotrophic Factor (BDNF). By dot blot, less than 1% cross-reactivity against NGF, NT3 or NT4.
Physical form
Format: Purified
Immunoglobulin fraction lyophilized from PBS, with no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
Preparation Note
Maintain lyophilized material at -20 to -70°C for up to one year after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Glycerol (ACS grade or better) can be added (1:1) for additional stability.
Other Notes
Replaces: AB1534P
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sensitized activation of Fos and brain-derived neurotrophic factor in the medial prefrontal cortex and ventral tegmental area accompanies behavioral sensitization to amphetamine.
Fanous S, Lacagnina MJ, Nikulina EM, Hammer RP Jr.
Neuropharmacology null
Aline Carolina Giardini et al.
Pain research & management, 2017, 7429761-7429761 (2017-04-20)
Background. Glial cells are implicated in the development of chronic pain and brain-derived neurotropic factor (BDNF) released from activated microglia contributes to the nociceptive transmission. Neural mobilization (NM) technique is a method clinically effective in reducing pain sensitivity. Here we
E M Nikulina et al.
Neuroscience, 212, 38-48 (2012-04-24)
Intermittent social defeat stress exposure augments behavioral response to psychostimulants in a process termed cross-sensitization. Brain-derived neurotrophic factor (BDNF) mediates synaptic plasticity and cellular responses to stress and drugs of abuse. We previously showed that repeated social defeat stress persistently
Chronic ketamine administration modulates midbrain dopamine system in mice.
Tan, S; Lam, WP; Wai, MS; Yu, WH; Yew, DT
Testing null
BDNF overexpression in the ventral tegmental area prolongs social defeat stress-induced cross-sensitization to amphetamine and increases ?FosB expression in mesocorticolimbic regions of rats.
Wang, J; Fanous, S; Terwilliger, EF; Bass, CE; Hammer, RP; Nikulina, EM
Neuropsychopharmacology null
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