ECM675
3D Collagen Culture Kit
3D cell culture kit includes: Human Collagen, Mediums (RPMI, M199 and DMEM), PBS with Phenol Red and Neutralization Solution
Synonym(s):
3D Cell Culture Kit, Collagen Culture Kit, Kit for 3D Collagen Culture
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1 KIT
CZK 12,400.00
About This Item
UNSPSC Code:
12352202
NACRES:
NA.75
eCl@ss:
32161000
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Product Name
3D Collagen Culture Kit, 3D cell culture kit includes: Human Collagen, Mediums (RPMI, M199 and DMEM), PBS with Phenol Red and Neutralization Solution
biological source
rat
form
liquid
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
activity assay: suitable
cell based assay: suitable
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
Quality Level
1 of 4
This Item | C6745 | 11179179001 | CC170 |
|---|---|---|---|
| biological source rat | biological source human cell culture | biological source rat tail | biological source Porcine |
| technique(s) activity assay: suitable, cell culture | mammalian: suitable, cell based assay: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level - |
| form liquid | form solution | form lyophilized (clear, colorless solution after reconstitution) | form - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in dry ice | shipped in - | shipped in ambient |
Application
Research Category
Cell Structure
Cell Structure
This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.
As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Cell migration is a fundamental function of normal cellular processes, including cell proliferation, and migration. Cell behavior in vitro is typically examined in a 2D environment; however, in the body, cells exist in a three-dimensional extracellular matrix environment rich in type I collagen. In fact, cells cultured in 3D matrices better reflect in vivo cell physiology when compared to traditional 2D systems.
CHEMICON (EMD Millipore) has developed a three-dimensional cell culture system that closely simulates the collagen-rich extracellular matrix of normal tissue. This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.
As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
For Research Use Only; Not for use in diagnostic procedures
CHEMICON (EMD Millipore) has developed a three-dimensional cell culture system that closely simulates the collagen-rich extracellular matrix of normal tissue. This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.
As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.
For Research Use Only; Not for use in diagnostic procedures
Other Notes
Collagen Solution - (Part No. 90135) One bottle - 10 mL
5X RPMI Medium- (Part No. 90134) One bottle - 2.5 mL
5X M199 Medium- (Part No. 90133) One bottle - 2.5 mL
5X DMEM Medium- (Part No. 90137) One bottle - 2.5 mL
5X PBS with Phenol Red- (Part No. 90136) One bottle - 2.5 mL
Neutralization Solution- (Part No. 90138) One vial - 0.5 mL
5X RPMI Medium- (Part No. 90134) One bottle - 2.5 mL
5X M199 Medium- (Part No. 90133) One bottle - 2.5 mL
5X DMEM Medium- (Part No. 90137) One bottle - 2.5 mL
5X PBS with Phenol Red- (Part No. 90136) One bottle - 2.5 mL
Neutralization Solution- (Part No. 90138) One vial - 0.5 mL
Physical form
Collagen solution is supplied in 6mM acetate buffer at ~ pH7.0.
Preparation Note
Store kit materials at 4°C up to their expiration date.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A
Storage Class
3 - Flammable liquids
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