MABC573

Anti-Bcl-2 Antibody, clone 1D7.2

clone 1D7.2, from rat

• Synonym(s): Apoptosis regulator Bcl-2, Bcl-2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 1D7.2, monoclonal
• Application: WB
• Citations: 3

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1D7.2, monoclonal
• species reactivity: mouse
• technique(s): western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_033871
• UniProt accession no.: P10417
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Bcl2(12043)

Description

General description

Apoptosis regulator Bcl-2 (UniProt P10417; also known as B-cell leukemia/lymphoma 2) is encoded by the Bcl2 (also known as Bcl-2, AW986256, C430015F12Rik, D630044D05Rik, D830018M01Rik) gene (Gene ID 12043) in murine species. Bcl-2 is an anti-apoptotic and the founding member of the Bcl-2 family of proteins that paly either a pro- or anti-apoptotic role in cell death signaling. BCL-2 family members are related by regions of sequence and structural homology. The pro-survival members contain four BCL-2 homology regions (BH1–BH4), whereas the BH3-only members contain only the BH3 amphipathic helix, which mediates their interaction with the groove of multi-domain BCL-2 family members. Human BCL2 gene mutatons are well known causes of many cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer.

~22 kDa observed (26.4 kDa/isoform 1 & 22.3 kDa/isoform 2, calculated).

Immunogen

Epitope: N-terminal half

GST-tagged recombinant protein corresponding to the N-terminal of mouse Bcl-2.

Application

Detect Bcl-2 using this rat Monoclonal Anti-Bcl-2 Antibody, clone 1D7.2, Cat. No. MABC573, validated for use in Western Blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

Expected to react with both spliced isoforms of murine Bcl-2.

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in mouse myoblast C2C12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Bcl-2 in 10 µg of mouse myoblast C2C12 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1