The concentration of this antibody is 1 mg/mL. The starting dilution factor is recommended at 1:2000 (0.5 µg/mL) for immunoblotting and 1:200 (5 µg/mL) for immunocytochemistry applications.
PC408
Anti-Green Fluorescent Protein (26-39) Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-GFP
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| Size/SKU | Availability | Price |
|---|---|---|
100 μL | Please contact Customer Service for Availability | 14 300,00 CZK |
About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
(GFP and some GFP fusions)
Application:
—
Citations:
3
biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
(GFP and some GFP fusions)
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:2000)
Immunocytochemistry (1:200))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Recognizes the ~28 kDa GFP and some GFP fusion proteins.
Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.
Immunogen
a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
Aequora victoria
Application
Immunoblotting (1:2000)
Immunocytochemistry (1:200)
Immunocytochemistry (1:200)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 1 mg/ml BSA.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.
This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
1 of 1
This Item | |||
|---|---|---|---|
| description liquid, Calbiochem® | description clone GFP-20, ascites fluid | description clone GSN24, purified from hybridoma cell culture | description ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| biological source rabbit | biological source mouse | biological source mouse | biological source rabbit |
| antibody form affinity isolated antibody | antibody form ascites fluid | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| species reactivity (GFP and some GFP fusions) | species reactivity - | species reactivity - | species reactivity - |
| clone polyclonal | clone GFP-20, monoclonal | clone GSN24, monoclonal | clone polyclonal |
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Storage Class
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Bonjour, Quelle est la concentration d'utilisation préconisée pour cet anticorps? Merci
1 answer-
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