SAB4200681

Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal

clone GFP-20, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse, green fluorescent protein
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: GFP-20, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 27 kDa
• species reactivity: rat, human
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 1-2 μg/mL using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein.; immunoprecipitation (IP): 5-10 μg using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein.
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal anti-green fluorescent protein (GFP) (mouse IgG1 isotype) is derived from the GFP-20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse BALB/c mice immunized with a GFP tagged fusion protein.GFP is a 27kDa protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca²⁺-activated photoprotein aequorin to GFP.

Immunogen

GFP tagged fusion protein

Application

Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse may be used in:

• immunoblotting[1]
• immunoprecipitation
• dot blot
• enzyme-linked immunosorbent assay (ELISA)
• scanning electron microscope (SEM)

Biochem/physiol Actions

GFP (green fluorescence protein) is a reporter molecule which is used for checking gene expression and protein localization. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells. GFP is considered as a unique tool to monitor dynamic processes in several living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence.

Monoclonal Anti-Green Fluorescent Protein (GFP) recognizes N-terminal and C-terminal GFP (27 kDa) tagged fusion proteins. The antibody reacts with fusion proteins expressed by prokaryotes expression vectors.

Physical form

a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Preparation Note

For continuous use, store at 28 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable