43107

Cyanocobalamin

tested according to Ph. Eur.

• Synonym(s): Vitamin B₁₂, α-(5,6-Dimethylbenzimidazolyl)cyanocobamide, CN-Cbl, Cyanocob(III)alamin, Cyanocobalamin
• Empirical Formula (Hill Notation): C₆₃H₈₈CoN₁₄O₁₄P
• CAS Number: 68-19-9
• Molecular Weight: 1355.37
• PubChem Substance ID: 329756307
• UNSPSC Code: 12352205
• MDL number: MFCD00151092
• NACRES: NA.21
• EC Number: 200-680-0
• Beilstein/REAXYS Number: 4122889

Properties

• Product Name: Cyanocobalamin, tested according to Ph. Eur.
• InChI key: RMRCNWBMXRMIRW-QJRSUKKJSA-L
• InChI: 1S/C62H90N13O14P.CN.Co/c1-29-20-39-40(21-30(29)2)75(28-70-39)57-52(84)53(41(27-76)87-57)89-90(85,86)88-31(3)26-69-49(83)18-19-59(8)37(22-46(66)80)56-62(11)61(10,25-48(68)82)36(14-17-45(65)79)51(74-62)33(5)55-60(9,24-47(67)81)34(12-15-43(63)77)38(71-55)23-42-58(6,7)35(13-16-44(64)78)50(72-42)32(4)54(59)73-56;1-2;/h20-21,23,28,31,34-37,41,52-53,56-57,76,84H,12-19,22,24-27H2,1-11H3,(H15,63,64,65,66,67,68,69,71,72,73,74,77,78,79,80,81,82,83,85,86);;/q;;+2/p-2/t31-,34-,35-,36-,37+,41-,52-,53-,56-,57?,59-,60+,61+,62+;;/m1../s1
• SMILES string: C/C(C1=N[C@]([C@]([C@H](CC(N)=O)[C@@]/2(C)CCC(NC[C@@H](OP([O-])(O[C@H]3[C@@H](O)[C@@H](N4C=[N]5C6=C4C=C(C)C(C)=C6)O[C@@H]3CO)=O)C)=O)([H])N([Co+]5C#N)C2=C(C)/C([C@@H](CCC(N)=O)C/7(C)C)=NC7=C/8)(C)[C@@](C)(CC(N)=O)[C@@H]1CCC(N)=O)=C9N=C8[C@@H](CCC(N)=O)[C@
• biological source: fermentation/recombinant
• Quality Level: 100
• agency: tested according to Ph. Eur.
• assay: 96.0-102.0% dry basis
• form: powder
• color: dark red
• solubility: water: soluble 1 gm in 80 ml; acetone: insoluble; alcohol: soluble; chloroform: insoluble; diethyl ether: insoluble
• application(s): cell analysis
• storage temp.: 2-8°C

Description

Application

Cyanocobalamin was added in the assay buffer during the analysis step of ELISA for ensuring that all transcobalamin was saturated with cobalamin before analysis.[1] Cyanocobalamin was also used for examining its impact on the expression of mPer1 and mPer2 in vivo.[2]

Biochem/physiol Actions

Cyanocobalamin plays a role in functioning of brain and nervous system. It is also involved in hematopoiesis. Vitamin B₁₂ deficiency leads to megaloblastic anemia. It is also associated with irreversible nervous system breakdown leading to spinal cord degeneration.[3]

Cyanocobalamin refers to a group of chemically-related cobalt containing molecules involved in cell processes such as DNA synthesis, fatty acid synthesis, energy production and regulation. Cyanocobalamin along with other B vitamins thiamine (B1) and pyridoxine (B6) blocks the thermal hyperalgesia in rats with neuropathic pain caused by spinal ganglia compression (CCD) or loose ligation of the sciatic nerve (CCI).[4] It can stimulate the anaerobic biodegradation of chloroform.

General description

Cyanocobalamin, also referred to as Vitamin B₁₂, is a water soluble vitamin.[3]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• ppe: Eyeshields, Gloves, type N95 (US)