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N2125

Naphthol AS-BI phosphate

≥98% purity (HPLC), powder

Naphthol AS-BI phosphate

Synonym(s):

7-Bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate

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CZK 72,200.00

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About This Item

Empirical Formula (Hill Notation):
C18H15BrNO6P
CAS Number:
1919-91-1
Molecular Weight:
452.19
NACRES:
NA.47
PubChem Substance ID:
24897552
UNSPSC Code:
12171500
EC Number:
217-645-0
MDL number:
MFCD00004058
Beilstein/REAXYS Number:
6668526

Key Documents

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Product Name

Naphthol AS-BI phosphate, Technical grade, ≥98% (HPLC)

InChI key

HUXIAXQSTATULQ-UHFFFAOYSA-N

InChI

1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)

SMILES string

COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O

assay

≥98% (HPLC)

form

powder

technique(s)

microbe id | staining: suitable

color

white to light yellow

solubility

methanol: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

200

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N2250N5625N4006
Naphthol AS-BI phosphate Technical grade, ≥98% (HPLC)

Sigma-Aldrich

N2125

Naphthol AS-BI phosphate

Naphthol AS-BI phosphate disodium salt hydrate ≥96.0% (HPLC)

Sigma-Aldrich

N2250

Naphthol AS-BI phosphate disodium salt hydrate

Naphthol AS phosphate >99% (TLC), histochemical substrate

Sigma-Aldrich

N5625

Naphthol AS phosphate

Naphthol AS-BI N-acetyl-β-D-glucosaminide β-hexosaminidase substrate

Sigma-Aldrich

N4006

Naphthol AS-BI N-acetyl-β-D-glucosaminide

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

-

technique(s)

-

color

white to light yellow

color

-

color

-

color

-

form

powder

form

powder

form

powder

form

powder

solubility

methanol: 50 mg/mL

solubility

water: 50 mg/mL, clear, colorless to yellow

solubility

ethanol: 50 mg/mL, clear, light yellow

solubility

DMF: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

assay

≥98% (HPLC)

assay

≥96.0% (HPLC)

assay

>99% (TLC)

assay

≥97% (TLC)

Application

Naphthol AS-BI phosphate is suitable to use:
  • in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
  • as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
  • to perform alkaline phosphatase reaction
Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.

Biochem/physiol Actions

Naphthol AS-BI can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

General description

Naphthol AS-BI, also known as 7-Bromo-3-hydroxy-2-naphtho-o-anisidine is a substituted naphthol.
Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM9911
BCCK2119
BCCD2606
BCBS3419V
BCBS3419

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A J Janckila et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 16(4), 788-793 (2001-04-24)
Tartrate-resistant acid phosphatase (TRAP) isoform 5b is a potential serum marker for osteoclastic activity. Biochemical assays for serum TRAP activity with para-nitrophenylphosphate (pNPP) have low specificity for bone because of hydrolysis by unrelated nontype 5 TRAPs of blood cells and
M Kimura et al.
Histochemistry, 82(6), 519-523 (1985-01-01)
A cytochemical study of acid phosphatase (AcP-ase) in the lateral prostate of the rat was performed to investigate whether AcP-ase in the secretory apparatus can be distinguished from AcP-ase in lysosomes and their related structures. Two types of AcP-ase were
Anthony J Janckila et al.
Clinica chimica acta; international journal of clinical chemistry, 347(1-2), 157-167 (2004-08-18)
Our purpose was to develop a specific immunoassay for tartrate-resistant acid phosphatase (TRACP) 5b using naphthol ASBI phosphate (N-ASBI-P) as a selective substrate for isoform 5b and heparin as a selective inhibitor of isoform 5a. Serum TRACP 5a and 5b
A M Boesen
Scandinavian journal of haematology, 32(3), 245-252 (1984-03-01)
The subcellular localization of acid phosphatase (AcP) in various immunologically-defined neoplastic lymphoid cells including hairy cells was investigated by electron microscopy. 2 substrates, naphthol-AS-BI-phosphoric acid (naphthol-AS-BI-P) and sodium beta-glycerophosphate, were compared. By incubation in naphthol-AS-BI-P containing medium, the reaction product
Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. I. Meijer's method.
P J Stoward et al.
Histochemistry, 71(3), 405-431 (1981-01-01)
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