Ponceau S is not recommended for gel staining; it is advised only for use with membranes. Using Ponceau S in acetic acid may precipitate and fix the proteins in the gel, which is not advisable if a protein transfer to a membrane is planned for a western blot.
P7170
Ponceau S solution
BioReagent, 0.1 % (w/v) in 5% acetic acid
Synonym(s):
Ponceau S
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1 L
CZK 5,660.00
About This Item
Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
Molecular Weight:
760.57
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
MDL number:
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Product Name
Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid
SMILES string
[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
InChI key
KQHKSGRIBYJYFX-WNDMICSESA-J
InChI
1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
description
for protein analysis
product line
BioReagent
form
liquid
concentration
0.1 % (w/v) in 5% acetic acid
technique(s)
western blot: suitable
density
1.01 g/cm3 at 20 °C
suitability
suitable (for use in cellulose acetate electrophoresis)
suitable for electrophoresis
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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This Item | P3504 | 1.14275 | 141194 |
|---|---|---|---|
| suitability suitable (for use in cellulose acetate electrophoresis), suitable for electrophoresis | suitability - | suitability - | suitability - |
| grade for protein analysis | grade - | grade - | grade - |
| technique(s) western blot: suitable | technique(s) electrophoresis: suitable | technique(s) - | technique(s) microbe id | staining: suitable |
| concentration 0.1 % (w/v) in 5% acetic acid | concentration - | concentration - | concentration - |
| form liquid | form powder | form solid | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing |
General description
Ponceau S is the sodium salt of a diazo dye.[4] Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting).[4] A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Protocols
Protokol Western blot podrobně popisuje přenos proteinů z gelů na nitrocelulózu, který je klíčový pro postupy imunoblottingu ve výzkumu.
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
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Is it practical to stain protein gels directly with Ponceau S without transferring to membranes in order to assess the amount of protein and number of bands in a protein sample? Is there a direct gel staining protocol available for this purpose?
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