A9186 is intended to demonstrate glycoproteins when performing electrophoresis, while A3157 is intended for use with tissue sections. It is then tested on paraffin sections of small intestine, trachea, and larynx using different methods. Since A9186 was not tested for use with tissue sections, it is possible that it might not stain as well as product A3157, which was performance tested on paraffin sections. The same principle applies to other Alcian Blue 8GX products that are not tested for specific applications. It is advised to try using A9186, as the staining may be acceptable. If the staining is not acceptable, it is recommended to consider ordering A3157.
A3157
Alcian Blue 8GX
Dye content, ≥50%, certified by the Biological Stain Commission, powder
Synonym(s):
Alcian Blue, Ingrain Blue 1
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Select a Size
10 G
CZK 8,980.00
25 G
CZK 17,700.00
About This Item
Empirical Formula (Hill Notation):
C56H68Cl4CuN16S4
CAS Number:
Molecular Weight:
1298.86
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
251-705-7
MDL number:
Colour Index Number:
74240
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Product Name
Alcian Blue 8GX, certified by the BSC
InChI key
CKLBXIYTBHXJEH-UHFFFAOYSA-J
InChI
1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4
SMILES string
[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C
agency
certified by the BSC
form
powder
composition
Dye content, ≥50%
technique(s)
microbe id | staining: suitable
color
dark blue
solubility
water: 1 mg/mL, blue
εmax
195-400 at 617-630 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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This Item | A5268 | 1.04136 | 335630 |
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) microbe id | staining: suitable |
| grade certified by the Biological Stain Commission | grade - | grade - | grade - |
| color dark blue | color blue | color - | color - |
| solubility water: 1 mg/mL, blue | solubility H2O: 1 mg/mL | solubility 8 g/L | solubility water: 1 mg/mL |
| form powder | form powder | form powder | form powder or crystals |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing () | application(s) diagnostic assay manufacturing |
Application
Alcian Blue 8GX has been used for the staining of sulfated proteoglycans in murine MPC (mesenchymal progenitor cell) nodule.[1] It has been used for the staining of sulfated proteoglycans in bovine articular chondrocytes.[2]
Certified for use in staining in Mowry′s pH 2.5 method, in Scott′s pH 5.7 method, and in Kreyberg′s method for keratin and mucin.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
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Can the A9186 Alcian blue 8GX be used for the same assay as the A3157 Alcian blue 8GX previously used for mice spinal cord histology (frozen sections)?
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