A9570

MilliShot^® Amphotericin B Ready Made Solution

250 μg/mL in deionized water, 0.2 μm filtered, BioReagent, suitable for cell culture

• Synonym(s): Amphotericin, Fungizone
• NACRES: NA.76

Properties

• biological source: yeast
• Quality Segment: 300
• sterility: 0.2 μm filtered
• product line: BioReagent
• form: liquid
• shelf life: 2 yr
• storage condition: protect from light
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

Amphotericin B is a macrocyclic lactone with a hydrophilic polyhydroxyl chain and a lipophilic polyene based hydrocarbon chain

Application

Amphotericin B solution has been used:

• as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice
• as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries
• as a constituent of transport medium during the collection of equine corpora lutea
• as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line

Biochem/physiol Actions

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium. Amphotericin B is most effective against fungi and yeast. It forms transmembrane channels by binding to sterols associated with membranes, thereby leading to the leakage of small molecules. Amphotericin B elicits toxicity despite being an effective antibiotic for treating mycoses.

Legal Information

MILLISHOT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable