Gamborg's B-5 Basal Medium with Minimal Organics is a plant tissue culture medium that contains reduced amounts of organic compounds compared to the standard Gamborg's B-5 Basal Medium. The minimal organics version is designed to provide a lower concentration of organic compounds, such as vitamins or amino acids, which allows for more precise control over nutrient supplementation in plant tissue culture experiments. Please see the link below to review the formulation tables for Gamborg's basal salt mixtures, products G5893 and G5768.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/186/351/g5768for.pdf
G5893
Gamborg′s B-5 Basal Medium with Minimal Organics
fine powder, suitable for plant cell culture
Gamborg’s B-5 Basal Medium with Minimal Organics
Synonym(s):
B-5 Basal Medium, Basal Medium, Minimal Organics Medium
Sign In to View Organizational & Contract Pricing
Select a Size
1 L
CZK 566.00
10 L
CZK 2,970.00
About This Item
UNSPSC Code:
12352207
NACRES:
NA.72
Skip To
form
fine powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
1 of 4
This Item | G5768 | M0404 | M9274 |
|---|---|---|---|
| technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | plant: suitable |
| form fine powder | form fine powder | form powder | form powder |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.[1]
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Application
Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
- as a component of liquid media for the tissue culture of Brassica rapa[2]
- as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings[3]
- as a component of solid GM medium for germination of Arabidopsis seeds[4]
Preparation Note
Formulated to contain 3.2 grams of powder per liter of medium.
With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Ox. Sol. 3
Storage Class Code
5.1B - Oxidizing hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
EU REACH Annex XVII (Restriction List)
CAS No.
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Establishment of Hairy Root Cultures for the Production of Biopharmaceuticals and Optimization of Methods for Recombinant Protein Secretion in the Culture Medium
Habitation (Elmsford, N.Y.), 153-173 (2020)
Leigh Mickelson-Young et al.
Journal of experimental botany, 67(21), 6077-6087 (2016-11-05)
The duration of the DNA synthesis stage (S phase) of the cell cycle is fundamental in our understanding of cell cycle kinetics, cell proliferation, and DNA replication timing programs. Most S-phase duration estimates that exist for plants are based on
Jakob Petereit et al.
Frontiers in plant science, 8, 72-72 (2017-02-24)
In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown
Experiments in Plant Tissue Culture (1985)
Rebeca Gonzalez-Bayon et al.
Plant physiology, 180(1), 240-252 (2019-02-03)
Hybrids are used extensively in agriculture due to their superior performance in seed yield and plant growth, yet the molecular mechanisms underpinning hybrid performance are not well understood. Recent evidence has suggested that a decrease in basal defense response gene
Articles
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
Related Content
-
What do you mean by minimal organics?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service