The product is cited in the reference Sulaimana, Saba A.J., et al., J of Enz Inhib and Med Chem. 18(4), 297-301 (2003).
G7509
GABase from Pseudomonas fluorescens
lyophilized powder, Protein ≥30 % by biuret
Synonym(s):
GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase
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10 UNITS
CZK 17,800.00
25 UNITS
CZK 28,300.00
About This Item
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Product Name
GABase from Pseudomonas fluorescens, lyophilized powder, Protein ≥30 % by biuret
biological source
Pseudomonas fluorescens
assay
≥30 % (biuret)
form
lyophilized powder
specific activity
≥0.5 units/mg protein
composition
Protein, ≥30% biuret
technique(s)
inhibition assay: suitable
storage temp.
−20°C
Quality Level
1 of 4
This Item | S2563 | A6306 | P0184 |
|---|---|---|---|
| assay ≥30 % (biuret) | assay - | assay - | assay - |
| biological source Pseudomonas fluorescens | biological source - | biological source bacterial (Pseudomonas atlantica) | biological source - |
| technique(s) inhibition assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form - | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
Application
Biochem/physiol Actions
GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase.[3]
General description
Other Notes
One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C.
Physical form
Partially purified lyophilized powder containing buffer salts and stabilizer
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Mingu Gordon Park et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 2016-2024 (2021-09-14)
Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification have not been established. Thus, biochemical and drug
Kimon-Andreas G Karatzas et al.
Applied and environmental microbiology, 76(11), 3529-3537 (2010-04-20)
It is well established that the glutamate decarboxylase (GAD) system is central to the survival of Listeria monocytogenes at low pH, both in acidic foods and within the mammalian stomach. The accepted model proposes that under acidic conditions extracellular glutamate
Structural and Mechanistic Basis for the Inactivation of Human Ornithine Aminotransferase by (3 S,4 S)-3-Amino-4-fluorocyclopentenecarboxylic Acid
Shen S, et al.
Molecules (Basel), 28(3) (2023)
Nicolaie Moldovan et al.
Sensors and actuators. B, Chemical, 337 (2022-05-24)
Glutamate (GLU) and gamma-aminobutyric acid (GABA) are neurotransmitters (NTs) with an essential role in signal transmission in the brain. Brain disorders, such as epilepsy, Alzheimer's and Parkinson's diseases, and traumatic brain injury can be linked to imbalances in the GLU-GABA
A Vidal-Cros et al.
The Biochemical journal, 229(3), 675-678 (1985-08-01)
L-threo-3-Fluoroglutamate and L-erythro-3-fluoroglutamate were tested with glutamate decarboxylase from Escherichia coli. Both isomers were substrates: the threo isomer was decarboxylated into optically active 4-amino-3-fluorobutyrate, whereas the erythro isomer lost the fluorine atom during the reaction, yielding succinic semialdehyde after hydrolysis
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Are there any literature references that cite use of the Product No. G7509, GABase from Pseudomonas fluorescens?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I solubilize Product No. G7509, GABase from Pseudomonas fluorescens?
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Sigma recommends to dissolve the product with 75 mM phosphate buffer, pH 7.2, containing 25% (v/v) glycerol to a concentration of 2-5 units per mL. Keep the solution in an ice bath while using and store solution frozen. This solution may be stored frozen and thawed several times with only slight loss of activity.
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What is the shelf life of the Product No.G7509, GABase from Pseudomonas fluorescens?
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Sigma recommends retesting the product 3 years after its release date. The retest date will appear on the lot specific Certificate of Analysis for the product.
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