The image below is stained with Brilliant Blue R-250
L4631
Lysozyme from chicken egg white
For use as a marker in SDS-PAGE
Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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1 VIAL
CZK 6,360.00
CZK 6,360.00
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
235-747-3
MDL number:
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Product Name
Lysozyme from chicken egg white, For use as a marker in SDS-PAGE
biological source
chicken egg white
form
powder
mol wt
single-chain 14.3 kDa
packaging
vial of 25 mg
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
chicken ... LYZ(396218)
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Related Categories
1 of 4
This Item | 62971 | 62970 | L3790 |
|---|---|---|---|
| biological source chicken egg white | biological source chicken egg white | biological source chicken egg white | biological source chicken egg white |
| Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) |
| form powder | form powder (crystalline) | form powder | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt single-chain 14.3 kDa | mol wt single-chain 14.3 kDa, Mr ~14600 | mol wt single-chain 14.3 kDa, Mr ~14600 | mol wt single-chain 14.3 kDa |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
Application
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Other Notes
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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