This product does not contain a tag.
P7837
Protein A from Staphylococcus aureus
Soluble, Cowan strain, recombinant, expressed in E. coli, aqueous solution, ≥95% (HPLC)
Skip To
Product Name
Protein A from Staphylococcus aureus, Soluble, Cowan strain, recombinant, expressed in E. coli, aqueous solution, ≥95% (HPLC)
recombinant
expressed in E. coli
conjugate
unconjugated
assay
≥95% (HPLC)
form
aqueous solution
mol wt
45,000
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
Staphylococcus aureus subsp. aureus NCTC 8325 ... SAOUHSC_00069(3919448)
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | P6031 | 82526 | 11412272001 |
|---|---|---|---|
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant expressed in CHO cells |
| assay ≥95% (HPLC) | assay - | assay - | assay >95% (SDS-PAGE) |
| form aqueous solution | form essentially salt-free, lyophilized powder | form powder | form solution |
| mol wt 45,000 | mol wt - | mol wt - | mol wt 25,000 Da |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Gene Information Staphylococcus aureus subsp. aureus NCTC 8325 ... SAOUHSC_00069(3919448) | Gene Information Staphylococcus aureus subsp. aureus NCTC 8325 ... SAOUHSC_00069(3919448) | Gene Information Staphylococcus aureus subsp. aureus NCTC 8325 ... SAOUHSC_00069(3919448) | Gene Information human ... TGFB1(7040) |
Application
Protein A may be conjugated with various reporter molecules including fluorescent dyes (FITC), enzyme markers (peroxidase, β-galactosidase, alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including western blotting, immunohistochemistry, and ELISA applications. In addition, protein A may be immobilized on a solid support such as agarose or acrylic beads for the purification of either polyclonal or monoclonal immunoglobulins. It is also routinely used for immunoprecipitation assays.
Biochem/physiol Actions
A highly stable cell surface receptor produced by several strains of Staphylococcus aureus.
Protein A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. It consists of a single polypeptide chain of molecular weight 42 kDa, containing four repetitive domains rich in aspartic and glutamic acids but devoid of cysteine. It contains little or no carbohydrate and only 4 tyrosine residues and no tryptophans. Protein A is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. One protein A molecule has been shown to bind at least 2 molecules of IgG simultaneously. The IgG binding domain of Protein A consists of three anti-parallel α-helicies, the third of which is disrupted when the protein is complexed with the Fc region of the immunoglobulins. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a, and IgG2b. Protein A also binds IgGs from other species, including monkey, rabbit, pig, guinea pig, dog, and cat.
Protein A also participates in a number of different protective biological functions including anti-tumor, toxic, and carcinogenic activities. In addition to acting as an immunomodulator, it also has antifungal and antiparasitic properties.
Protein A also participates in a number of different protective biological functions including anti-tumor, toxic, and carcinogenic activities. In addition to acting as an immunomodulator, it also has antifungal and antiparasitic properties.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Chaitali P Basole et al.
Cellular signalling, 40, 62-72 (2017-08-31)
The levels of cAMP are regulated by phosphodiesterase enzymes (PDEs), which are targets for the treatment of inflammatory disorders. We have previously shown that PDE8 regulates T cell motility. Here, for the first time, we report that PDE8A exerts part
Thierry Champion et al.
Methods in molecular biology (Clifton, N.J.), 988, 331-343 (2013-03-12)
Surface plasmon resonance (SPR) is a key technology to evaluate IgG effector functions in vitro involving binding to Fcgamma receptors (FcγR). We describe here the chemical coupling of protein A to a sensor chip. IgGs prepared either from purified antibodies
Irene Tom et al.
Current protocols in protein science, Chapter 27, Unit 27-Unit 27 (2013-04-03)
Functional protein microarrays offer the capability for high-throughput protein interaction analysis and have long promised to be a powerful tool for understanding protein interactions at the proteome scale. Although popular techniques for protein-protein interaction mapping like yeast-two-hybrid and affinity-purification mass
Microplate-based chromatin immunoprecipitation method, Matrix ChIP: a platform to study signaling of complex genomic events.
Flanagin S et al
Nucleic Acids Research, 36(3), e17-e17 (2008)
Sally L Sherwen et al.
Zoo biology, 34(4), 287-295 (2015-06-04)
Free range exhibits are becoming increasingly popular in zoos as a means to enhance interaction between visitors and animals. However very little research exists on the impacts of visitors on animal behaviour and stress in free range exhibits. We investigated
-
Does this product have a tag?
1 answer-
Helpful?
-
-
What is the concentration of this product? Is it 1 mg/mL? Thanks
1 answer-
The concentration of this product varies from lot to lot. The minimum protein concentration is 50 mg/mL and the minimum purity is 95%. At the minimum concentration and purity, the 5 mg package will contain 105.2 uL. The lot specific values are listed in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/p7837#product-documentationHelpful?
-
-
What is the solvent for this product?
1 answer-
The solvent for this product is deionized water, and no buffer is added.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service