P9172

Pyronin Y

for NA electrophoresis

• Synonym(s): Pyronin Y, Pyronin G, Pyronin J
• Empirical Formula (Hill Notation): C₁₇H₁₉ClN₂O
• CAS Number: 92-32-0
• Molecular Weight: 302.80
• Colour Index Number: 45005
• Beilstein: 3795789
• EC Number: 202-147-8
• MDL number: MFCD00011725
• UNSPSC Code: 12171500
• PubChem Substance ID: 57654569
• NACRES: NA.52

Properties

• grade: Molecular Biology
• form: powder
• composition: Dye content, ≥45%
• technique(s): nucleic acid detection: suitable
• mp: 250-260 °C (lit.)
• solubility: water: 10 mg/mL, dark red
• suitability: suitable for nucleic acid staining
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
• InChI: 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
• InChI key: INCIMLINXXICKS-UHFFFAOYSA-M

Description

Application

Pyronin Y has been used:

• in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)[1]
• as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)[2]
• staining of CD34⁺ cells[3]

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells.[4] In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves