R4752

Ribonucleic acid, transfer from bovine liver

Type XI, lyophilized powder

• Synonym(s): Transfer RNA, tRNA
• CAS Number: 9014-25-9
• MDL number: MFCD00132200
• UNSPSC Code: 41106305
• NACRES: NA.51

Properties

• type: Type XI
• Quality Level: 200
• form: lyophilized powder
• color: white to light yellow
• suitability: suitable for (gives a positive response when incubated at pH 7.6 at 37°C with aminoacyl-tRNA synthetase and the amino acid L-arginine)
• storage temp.: −20°C

Description

General description

Transfer ribonucleic acid (tRNA) is a non-coding RNA. It is approximately 70-100 base pair long. It has a cloverleaf shaped secondary structure.[1] It has three or four arms and a stem structure. It has minor bases like pseudouridine, dihydrouridine and methylated bases in its structure. It has a binding site for triplet nucleotide sequence and for amino acids.[2]

Application

Ribonucleic acid, transfer from bovine liver has been used:

• to analyze the efficiency of thioflavin T (ThT) as a fluorophore[3]
• to test its ability to inhibit the growth of endothelial cells[4]
• as a component in translation mixture[5]
• as a non-specific competitor RNA for protein purification studies using poly(A) tailed RNA affinity column[6]

Ribonucleic acid, transfer from bovine liver may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).

Biochem/physiol Actions

Transfer ribonucleic acid (tRNA) plays a major role in translation, a key step in protein synthesis. It decodes mRNA into protein.[1] There are about 40-60 different tRNAs in a eukaryotic cell and fewer in bacteria. This depends on the degeneracy of the amino acid code. Each amino acid has a specific tRNA. tRNAs are charged with specific amino acid and carries them to the site of protein synthesis. It binds to the ribosome and directs the amino acid to the growing polypeptide chain.[2] It also functions as a regulator of various biological processes. Mutations associated with tRNA is implicated in human diseases.[1]

Analysis Note

This product is evaluated for amino acceptor activity using aminoacyl-tRNA synthetase and L-arginine

Other Notes

One unit will yield an A₂₆₀ of 1.0 in 1.0 mL of water (1 cm light path).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves