SML2275

TH-257

≥98% (HPLC)

• Synonym(s): N-Benzyl-N-butyl-4-(N-phenylsulfamoyl)benzamide, N-Butyl-4-[(phenylamino)sulfonyl]-N-(phenylmethyl)benzamide
• Empirical Formula (Hill Notation): C₂₄H₂₆N₂O₃S
• CAS Number: 2244678-29-1
• Molecular Weight: 422.54
• UNSPSC Code: 12352200
• NACRES: NA.77
• Assay: ≥98% (HPLC)
• Form: powder

Properties

• assay: ≥98% (HPLC)
• form: powder
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: 2-8°C
• SMILES string: O=C(C1=CC=C(C=C1)S(NC2=CC=CC=C2)(=O)=O)N(CC3=CC=CC=C3)CCCC
• InChI: 1S/C24H26N2O3S/c1-2-3-18-26(19-20-10-6-4-7-11-20)24(27)21-14-16-23(17-15-21)30(28,29)25-22-12-8-5-9-13-22/h4-17,25H,2-3,18-19H2,1H3
• InChI key: VNCIWNGCMAKKEO-UHFFFAOYSA-N

Description

Biochem/physiol Actions

Potent, type III allosteric LIM-kinase (LIMK1 & LIMK2) inhibitor that targets LIMK1/2 DFG-out conformation in a non-ATP-competitive manner.

TH-257 is a potent, type III allosteric LIM-kinase (LIMK1 & LIMK2) inhibitor (LIMK1/2 IC50 in nM = 48/29 by cell-free kinase assay and 250/150 by live cell NanoBRET assay using respective transfectants) that targets LIMK1/2 DFG-out conformation in a non-ATP-competitive manner and exhibits no significant off-target activity by KINOMEscan assay at 1 μM. TH-263 is a structure analog and the recommended negative control for TH-257. For characterization details of TH-257, please visit the TH-257 probe summary on the Structural Genomics Consortium (SGC) website.

TH-263 is the negative control for the active probe, TH-257. TH-263 is available from Sigma. To learn more about and purchase TH-263, click here.

To learn about other SGC chemical probes, visit sigma.com/sgc

Features and Benefits

TH-257 is a chemical probe available through a partnership with the Structural Genomics Consortium (SGC). To learn more and view other SGC epigenetic probes, visit sigma.com/SGC.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable