MABF80D

Anti-MHC class II (I-A/I-E), FITC, clone M5/114 .15.2 Antibody

clone M5/114.15.2, from rat, FITC conjugate

• Synonym(s): H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain (Ii), MHC class II-associated invariant chain, CD74, MHC class II (I-A/I-E), FITC
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• conjugate: FITC conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: M5/114.15.2, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• isotype: IgG2bκ
• UniProt accession no.: P04441
• target post-translational modification: unmodified
• Gene Information: mouse ... Cd74(16149)

Description

General description

The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Anti-MHC class II (I-A/I-E), FITC, clone M5/114 .15.2 Antibody is an antibody against MHC class II for use in Flow Cytometry.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected MHC class II (I-A/I-E), FITC in one million C57Bl/6 splenocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable