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Merck

RECYMAG65K27PMX

MILLIPLEX® Rat Cytokine/Chemokine Magnetic Bead Panel - Premixed 27 Plex - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in rat serum, plasma and cell culture samples.

Synonym(s):

luminex rat cytokine chemokine growth factor multiplex assay, luminex rat pro inflammatory anti inflammatory cytokine panel, millipore rat inflammation multiplex kit, rat interleukin immunoassay panel

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

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Quality Level

species reactivity

rat

manufacturer/tradename

Milliplex®

assay range

accuracy: 80-131%
standard curve range: 0.2-1,000 pg/mL
(EGF)

standard curve range: 1.2-5,000 pg/mL
(GRO/KC)

standard curve range: 12.2-50,000 pg/mL
(GM-CSF, IL-1α, IL-2, IL-12 (p70), IL-18)

standard curve range: 14.6-60,000 pg/mL
(IFNγ, MIP-1α)

standard curve range: 2.4-10,000 pg/mL
(Fractalkine, IP-10, Leptin, TNFα)

standard curve range: 24.4-100,000 pg/mL
(LIX, MIP-2)

standard curve range: 4.9-20,000 pg/mL
(Eotaxin, C-CSF, IL-4, IL-5, IL-13, RANTES, VEGF)

standard curve range: 7.3-30,000 pg/mL
(IL-10, IL-17A, MCP-1)

standard curve range: 73.2-300,000 pg/mL
(IL-6)

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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This Item
RECYMAG65PMX27BKCCYTMAG90KPX13BKHCD8MAG15K17PMX
species reactivity

rat

species reactivity

rat

species reactivity

canine

species reactivity

human

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 80-131%, standard curve range: 1.2-5,000 pg/mL
(GRO/KC), standard curve range: 14.6-60,000 pg/mL
(IFNγ, MIP-1α), standard curve range: 24.4-100,000 pg/mL
(LIX, MIP-2), standard curve range: 7.3-30,000 pg/mL
(IL-10, IL-17A, MCP-1), standard curve range: 0.2-1,000 pg/mL
(EGF), standard curve range: 2.4-10,000 pg/mL
(Fractalkine, IP-10, Leptin, TNFα), standard curve range: 73.2-300,000 pg/mL
(IL-6), standard curve range: 12.2-50,000 pg/mL
(GM-CSF, IL-1α, IL-2, IL-12 (p70), IL-18), standard curve range: 4.9-20,000 pg/mL
(Eotaxin, C-CSF, IL-4, IL-5, IL-13, RANTES, VEGF)

assay range

-

assay range

accuracy: 101%
(IL-8), accuracy: 102%
(IL-7), accuracy: 109%
(IL-15), accuracy: 95%
(GM-CSF), accuracy: 96%
(IL-18), accuracy: 98%
(IL-6), sensitivity: 13.6 pg/mL
(IFN-γ), sensitivity: 21.0 pg/mL
(MCP-1), sensitivity: 21.7 pg/mL
(IL-8), sensitivity: 3.2 pg/mL
(IP-10), sensitivity: 3.5 pg/mL
(IL-2), sensitivity: 3.7 pg/mL
(IL-6), sensitivity: 5.3 pg/mL
(KC-like), sensitivity: 5.8 pg/mL
(IL-18), sensitivity: 6.1 pg/mL
(TNFα), sensitivity: 7.5 pg/mL
(IL-7), sensitivity: 8.5 pg/mL
(IL-10), sensitivity: 9.0 pg/mL
(IL-15), sensitivity: 9.2 pg/mL
(GM-CSF), standard curve range: 12.2-50,000 pg/mL
(All analytes)

assay range

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

compatibility

configured for Premixed

compatibility

configured for Premixed

compatibility

configured for Premixed

compatibility

configured for Premixed

General description

The MILLIPLEX® Rat Cytokine/Chemokine Bead Panel (RECYTMAG-65K & RECYMAG65K27PMX) enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression.

Panel Type: Cytokines/Chemokines

Application

  • Analytes: EGF, Eotaxin/CCL11, Fractalkine, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, IP-10, Leptin, LIX, MCP-1, MIP-1α, MIP-2, RANTES, TNF-α, VEGF
  • Recommended Sample type: rat serum or plasma
  • Recommended Sample dilution: 1:2
  • Assay Run Time: One day

Biochem/physiol Actions

Cross Reactivty
There is no or minimum cross-reactivity between the antibodies and any other analytes in the panel.

Other Notes

Sensitivity: Refer to kit protocol for sensitivites for individual cytokines/chemokines

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

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Dominic Friston et al.
Disease models & mechanisms, 13(4) (2020-03-05)
Burn injury is a pathology underpinned by progressive and aberrant inflammation. It is a major clinical challenge to survival and quality of life. Although the complex local and disseminating pathological processes of a burn injury ultimately stem from local tissue
Young Taek Oh et al.
American journal of physiology. Endocrinology and metabolism, 309(2), E154-E160 (2015-05-21)
Inflammation is implicated in metabolic abnormalities in obesity and type 2 diabetes. Because θ-defensins have anti-inflammatory activities, we tested whether RTD-1, a θ-defensin, improves metabolic conditions in diet-induced obesity (DIO). DIO was induced by high-fat feeding in obese-prone CD rats
Norhashima Abd Rashid et al.
EXCLI journal, 19, 1246-1265 (2020-10-31)
Oxidative stress, inflammation and apoptosis are thought as primary mediators of cisplatin-induced hepatotoxicity. The objective of this study was to determine the protective effect of Polygonum minus essential oil in cisplatin-induced hepatotoxicity. A total of forty-two male rats were randomly
Natalia Mendez et al.
Frontiers in endocrinology, 10, 362-362 (2019-06-28)
Adverse prenatal conditions are known to impose significant trade-offs impinging on health and disease balance during adult life. Among several deleterious factors associated with complicated pregnancy, alteration of the gestational photoperiod remains largely unknown. Previously, we reported that prenatal manipulation
Linda W Zhang et al.
Frontiers in neuroscience, 10, 561-561 (2016-12-23)
Magnesium sulfate (MgSO4) is the most widely used therapy in the clinic to prevent the progression of preeclampsia, a hypertensive disorder of pregnancy, to eclampsia. Eclampsia, manifested as unexplained seizures and/or coma during pregnancy or postpartum, accounts for ~13% of

Questions

  1. Are all the standards in the Rat Cytokine Milliplex kit RECYTMAG-65K rat standards, and are they recombinant?

    1 answer
    1. All standards in the Rat Cytokine Milliplex kit RECYTMAG-65K are recombinant. They are all from rat except for G-CSF and IL-17A, which are from mouse, and Eotaxin, which is from human.

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