As per the Product Information Sheet, the Accutase solution does not contain mammalian or bacterial-derived products. This solution is supplied as a sterile, ready-to use, frozen liquid. This product is serum free.
A6964
Accutase® solution
sterile-filtered, suitable for cell culture
Synonym(s):
Cell detachment solution
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sterility
sterile-filtered
Quality Level
form
liquid
concentration
400-600 units/mL
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
impurities
≤1 EU/mL endotoxin
pH
6.8-7.8
shipped in
dry ice
storage temp.
−20°C
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This Item | SCR005 | SCR104 | A7089 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| form liquid | form liquid | form liquid | form solution |
| concentration 400-600 units/mL | concentration - | concentration - | concentration 1 × |
| pH 6.8-7.8 | pH - | pH - | pH 6.8-7.8 |
| sterility sterile-filtered | sterility - | sterility - | sterility sterile-filtered |
| storage temp. −20°C | storage temp. - | storage temp. - | storage temp. −20°C |
General description
Accutase® solution (in Dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion- coated plasticware. Accutase is preferred over trypsin for cell detachment as it helps to detach cells from culture vessels with less damage. Hence, Accutase is often used for the treatment of sensitive cells or for analyses of surface markers.
Application
Accutase® solution has been used for dissociation or digestion of nonadherent cells growing in an aggregated cluster, such as brain tumor cells,[1] induced pluripotent stem cells (IPSCs)[2] and glioblastoma cells[3] into single cells.
Suitable for use in preparation of single cell suspension for sequencing.
Suitable for use in preparation of single cell suspension for sequencing.
Proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Performs exceptionally well in detaching cells for analysis of cell surface markers, virus growth assay, and flow cytometry as well as bioreactor scale-up. Does not contain mammalian or bacterial-derived products.
Features and Benefits
Ready-to-use liquid consisting of a proprietary mixture of proteolytic and collagenolytic enzymes for use in dissociation of cells from standard and adhesion-coated plasticware.
Preparation Note
Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red. Refer to Product Data Sheet on the website for product usage information.
This product contains phenol red. The product is shipped on dry ice and there could be significant CO2 buildup in the package. This CO2 may enter the solution and lower the pH slightly giving a yellow-orange (around pH 6.8) color. The solution should still be good to use as is, or sodium hydroxide may be added to adjust the pH. A fully frozen bottle of Accutase will be yellow in color and change to an orange color as it defrosts. Once unpacked, the product can be refrozen or placed directly into the refrigerator for later use. Do not defrost the product in a warm water bath. Accutase is sensitive to temperatures above 37 °C and will be inactivated after 45 minutes at 37 °C. Defrosting overnight in the refrigerator or in a tub of cold tap water for 1-1.5 hours is recommended. Shake up the bottle of Accutase after defrosting to enable the ingredients to go into solution evenly. These products do not need to be warmed up before being used. Use promptly once removed from the refrigerator, and return to the refrigerator after use.
Legal Information
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Is Accutase Solution A6964 animal-origin free? Is serum free?
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Can accutase be thawed, aliquoted, and refrozen at -20 to preserve it longer?
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Yes, this product is stable after undergoing several freeze/thaw cycles. The thawed product may be aliquoted and stored at –20 °C or stored for up to 2 months at 4°C.
The graphic below demonstrates the freeze/thaw stability of Accutase.Helpful?
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On the Innovative cell technologies website it says that accumax is the same as accutase but with 3x the amount of enzymes. Why did the technician here answer that it contains DNAse?
1 answer-
Thank you for calling this to our attention. The manufacturer, Innovative Cell Technologies, has been contacted regarding this matter. The manufacturer has confirmed that both Accutase and Accumax products DO contain a small amounts of DNAse. The concentration is not measured, but it is present in the formulation. In addition, both products also contain 0.5 mM EDTA.
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What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?
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Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells. The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27
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How can I inactivate Accutase® with fetal bovine serum?
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Due to its gentle nature Accutase® cell detachment solution usually does not need to be neutralized following cell passaging. Accutase® is also heat inactivated after 45 minutes at 37°C. If this method of neutralization does not work for your cells, or if you want to neutralize Accutase® in a shorter period of time, the addition of 10% FBS containing media will accomplish this task. Experiments have shown that diluting a sample of Accutase® with 10% with fetal bovine serum achieved an environment similar to that found in cell culture. Measurements of the enzyme activity in this diluted sample showed decreased activity over time, falling below that of the required level for cell detachment (>175 units/mL). After about 6 minutes of serum interaction, the enzyme activity of Accutase® was well below this level, demonstrating effective inhibition by FBS.
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For what purposes can Product A6964, Accutase® solution, be used?
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It can be used for detaching cells, analysis of cell surface markers and virus growth assays.
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What is the concentration of Product A6964, Accutase® solution?
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This product is typically at a concentration of 400-600 units/mL.
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What is the difference between Accutase and Accumax?
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Accutase is a mixture of non-mammalian proteases. Accumax is accutase that has been augmented with DNase to reduce clumping associated with DNA release from damaged cells.
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Will Product A6964, Accutase® solution, work on tissue?
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This product is not tested for tissue, but it should work on soft tissue.
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Does Product A6964, Accutase® solution, contain animal components?
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Accutase is purified from a marine invertebrate. There are no other animal or bacterial derived proteins present.
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