B3687

Biliverdin Reductase A human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous solution

• Synonym(s): BLVRA, BVRA, Bilirubin: NAD(P)⁺ oxidoreductase
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥90% (SDS-PAGE)
• form: buffered aqueous solution
• specific activity: ≥700 units/mg protein
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... BLVRA(644)

Description

General description

Research area: IMMUNO AND CKS. Biliverdinreductase A (BVRA) is an isozyme of the biliverdin reductase. It is a cellsurface membrane receptor. BVRA consists of two major regions, theregulatory/DNA interaction domain and catalytic domain.[1]

Application

Biliverdin Reductase Ahuman has been used as a component of the incubation medium to evaluate theactivity of hemeoxygenase 1.[2] It has also been used as a standard todetermine the age of the bleed from haemorrhagic lesion.[3]

Biochem/physiol Actions

Biliverdin reductase catalyzes the transformation of the blue-green pigment biliverdin IX to the yellow-orange bile pigment bilirubin IX by converting a double-bond between the second and third pyrrole ring into a single-bond.[4] This enzyme has two distinct cofactor-dependent pH optima. In the acidic range of pH 6.0-6.7, NADH is utilized, whereas in the alkaline range of pH 8.5-8.7, NADPH is utilized.[5] Biliverdin reductase is considered a major physiologic cytoprotectant. Biliverdin reductase suppresses experimental autoimmune encephalomyelitis in rats.[6] Depletion of biliverdin reductase leads to accumulation of cellular oxidants and augmented cell death.[7]Biliverdin reductase (BVR)modulates the functions of insulin via the insulin-like growth factor 1 (IGF-1)pathway. It shows dual specificity kinases where it phosphorylates tyrosineapart from serine and threonine. BVR acts as a biomarker in certain cancers.[8]

Physical form

Solution containing 20 mM potassium phosphate buffer, 10% glycerol, 0.2% Igepal CA630, 1 mM EDTA, and 0.1 mM DTT.

Other Notes

1 unit of biliverdin reductase will trans­form 1 nanomole of biliverdin to bilirubin per minute in an NADPH dependent reaction at pH 8.5 using 100 mM K-phosphate buffer at 37 °C.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable