We recommend Product No. C0775 (Crystal Violet, certified by the Biological Stain Commission), or Product No. 93770 (Tuerk's solution) for these stains, rather than diluting down the relatively high concentration of Crystal Violet (2.3%) in HT901 to approximately 0.1%. Product HT901 was formulated for use in the Gram Stain procedure. The product's performance has not been tested for use in colony staining, migration or plaque assays.
HT901
Crystal Violet Solution
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About This Item
Empirical Formula (Hill Notation):
C25H30ClN3
CAS Number:
Molecular Weight:
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3580948
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InChI
1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
InChI key
ZXJXZNDDNMQXFV-UHFFFAOYSA-M
SMILES string
[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
form
solution
shelf life
Expiry date on the label
IVD
for in vitro diagnostic use
concentration
2.3%
technique(s)
microbe id | staining: suitable
pH
4.6
application(s)
hematology
histology
storage temp.
room temp
Quality Level
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1 of 4
This Item | HT90132 | V5265 | C0775 |
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| concentration 2.3% | concentration 2.3% | concentration 1% | concentration - |
| form solution | form solution | form aqueous solution | form powder |
| application(s) hematology | application(s) hematology | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| IVD for in vitro diagnostic use | IVD for in vitro diagnostic use | IVD - | IVD - |
| shelf life Expiry date on the label | shelf life Expiry date on the label | shelf life - | shelf life - |
Application
Kit component of Gram Stain kits HT90A and HT90T (for staining of heat-fixed films and tissue, respectively).
Other Notes
HT901 is composed of 2.3% certified crystal violet, 0.1% ammonium oxalate monohydrate, and 20% ethyl alcohol, SD3A.
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 2
flash_point_f
100.0 °F - closed cup
flash_point_c
37.8 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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W R Richter et al.
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Hepatology (Baltimore, Md.), 69(6), 2608-2622 (2019-01-30)
Replication fork stability during DNA replication is vital for maintenance of genomic stability and suppression of cancer development in mammals. ATR (ataxia-telangiectasia mutated [ATM] and RAD3-related) is a master regulatory kinase that activates the replication stress response to overcome replication
Hansjörg Hufnagel et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 17(8), 1411-1417 (2009-06-18)
The understanding of internalization pathways of lipo- or polyplexes is crucial for engineering successful reagents for nonviral gene transfection. A known inhibitor of fluid phase endocytosis (FPE), rottlerin, was used to quantify the contribution of this pathway by flow cytometric
Kai-Feng Hung et al.
International journal of biological sciences, 15(5), 1080-1090 (2019-06-12)
Up-regulation of ASB6 has been previously associated with late-stage and poor prognosis of oral squamous cell carcinoma (OSCC) patients. To explore the cellular and molecular basis of how ASB6 enhances the malignancy of OSCC, we employed the clonogenicity and migration
Danuta Martuszewska et al.
PloS one, 4(2), e4350-e4350 (2009-02-06)
The Tensin family of intracellular proteins (Tensin1, -2, -3 and -4) are thought to act as links between the extracellular matrix and the cytoskeleton, and thereby mediate signaling for cell shape and motility. Dysregulation of Tensin expression has previously been
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Is Product HT901, Crystal violet solution, suitable for colony staining, migration or plaque staining?
1 answer-
Helpful?
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What is the formulation of Product HT901, Crystal violet solution?
1 answer-
HT901 is composed of:certified Crystal Violet, 2.3%Ammonium Oxalate, 0.1%20% denatured ethyl alcohol.
Helpful?
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Is Product HT901, Crystal violet solution, used as part of a kit?
1 answer-
Although available separately, this solution is also a component of Gram Stain kits HT90A and HT90T (for staining of heat-fixed films and tissue, respectively).
Helpful?
-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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