400088

HIF-1α Translation Inhibitor, KC7F2

The HIF-1α Translation Inhibitor, KC7F2, also referenced under CAS 927822-86-4, controls the biological activity of HIF-1α. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Synonym(s): HIF-1α Translation Inhibitor, KC7F2, Hypoxia-Inducible Factor-1α Translation Inhibitor, KC7F2, N,Nʹ-(2,5-Dichlorosulfonyl)cystamine, HIF Inhibitor III
• Empirical Formula (Hill Notation): C₁₆H₁₆Cl₄N₂O₄S₄
• CAS Number: 927822-86-4
• Molecular Weight: 570.38
• MDL number: MFCD22683809
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: beige to tan
• solubility: DMSO: 100 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [S](=O)(=O)(NCCSSCCN[S](=O)(=O)c2c(ccc(c2)Cl)Cl)c1c(ccc(c1)Cl)Cl
• InChI: 1S/C16H16Cl4N2O4S4/c17-11-1-3-13(19)15(9-11)29(23,24)21-5-7-27-28-8-6-22-30(25,26)16-10-12(18)2-4-14(16)20/h1-4,9-10,21-22H,5-8H2
• InChI key: REQLACDIZMLXIC-UHFFFAOYSA-N

Description

General description

A cell-permeable symmetrical cystamine compound that selectively suppresses cellular HIF-1α, but not HIF-1β, protein synthesis without affecting the transcription of HIF-1α mRNA or the stability of HIF-1α protein. Reduction of cellular HIF-1α by KC7F2 treatment (IC₅₀<20 µM in LN229 cultures under hypoxia condition) is shown to result in decreased transcriptions of HIF-1α-dependent genes, such as CA IX, endothelin 1, enolase 1, and MMP-2. Inhibition of 4EBP1 and S6K phosphorylation, important event for the initiation of protein translation, is also observed in KC7F2 and U87MGD cultures following KC7F2 treatment under hypoxia condition, which may account for, at least in part, the downregulated HIF-1α synthesis.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Narita, T., et al. 2009. Clin. Cancer Res.15, 6128.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable