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Merck

B4261

Biocytin

≥98% (TLC), suitable for drug transporter assays

Biocytin

Synonym(s):

Bct, Nε-(+)-Biotinyl-L-lysine

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5 MG

433,00 kr.

25 MG

1.170,00 kr.

50 MG

2.000,00 kr.

100 MG

3.350,00 kr.

250 MG

6.660,00 kr.

433,00 kr.


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About This Item

Empirical Formula (Hill Notation):
C16H28N4O4S
CAS Number:
Molecular Weight:
372.48
NACRES:
NA.26
PubChem Substance ID:
UNSPSC Code:
12352209
MDL number:
Beilstein/REAXYS Number:
97197

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Product Name

Biocytin, ≥98% (TLC)

InChI

1S/C16H28N4O4S/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24)/t10-,11-,12-,14-/m0/s1

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCCC[C@H](N)C(O)=O)[C@@]1([H])NC(=O)N2

InChI key

BAQMYDQNMFBZNA-MNXVOIDGSA-N

assay

≥98% (TLC)

form

powder

optical activity

[α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)

technique(s)

drug transporter assay: suitable

color

white to off-white

mp

~245 °C (dec.) (lit.)

storage temp.

−20°C

Quality Level

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1 of 4

This Item
B1758A2010A3626
form

powder

form

powder

form

powder

form

powder

technique(s)

drug transporter assay: suitable

technique(s)

drug transporter assay: suitable

technique(s)

ligand binding assay: suitable

technique(s)

ligand binding assay: suitable

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥98% (TLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

optical activity

[α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)

optical activity

-

optical activity

-

optical activity

-

Application

Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. Biocytin has been used to label neurons, medium spiny neurons and optic nerves.[1][2][3]

Biochem/physiol Actions

Biocytin is an adduct of lysine and biotin.[4] It is hydrolyzed by biotinidase, which acts on peptide-incorporated biotin or free biotin.[5] Biocytin acts as a coenzyme.[1]

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Find documentation for the products that you have recently purchased in the Document Library.

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Extraocular Source of Oligodendrocytes Contribute to Retinal Myelination and Optokinetic Responses in Zebrafish
Chen Tian
Investigative Ophthalmology & Visual Science, 57(4) (2016)
Srinivasan Damodaran, Kirk L. Parkin, Owen R. Fennema
Fennema's Food Chemistry, Fourth Edition (2007)
Dbx1 precursor cells are a source of inspiratory XII premotoneurons
Ann L Revill
eLife, 4, e12301-e12301 (2015)
Treadmill exercise reverses dendritic spine loss in direct and indirect striatal medium spiny neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson?s disease
William A
Neurobiology of Disease (2014)
Cytometry (2000)

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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