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Merck

B9410

BIS-TRIS propane

BioXtra, ≥99.0% (titration)

Synonym(s):

1,3-Bis[tris(hydroxymethyl)methylamino]propane

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25 G

2.120,00 kr.

100 G

5.630,00 kr.

250 G

13.030,00 kr.

2.120,00 kr.


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About This Item

Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
Molecular Weight:
282.33
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
264-899-3
Beilstein/REAXYS Number:
1786109
MDL number:

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Product Name

BIS-TRIS propane, BioXtra, ≥99.0% (titration)

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

product line

BioXtra

assay

≥99.0% (titration)

form

powder

impurities

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

10.0-12.0 (1 M in H2O)

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.05 at 280 in H2O at 1 M
≤0.1 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

storage temp.

room temp

Quality Level

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1 of 4

This Item
B6755394111137269
assay

≥99.0% (titration)

assay

≥99.0% (titration)

assay

>98% (titration)

assay

-

product line

BioXtra

product line

-

product line

-

product line

EMPROVE® EVOLVE

Quality Level

400

Quality Level

400

Quality Level

100

Quality Level

400

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing
general analytical
life science and biopharma

application(s)

-

application(s)

-

form

powder

form

powder

form

solid

form

powder

solubility

H2O: 1 M, clear, colorless

solubility

water: 30 % (w/w), clear, colorless

solubility

water: 1 M

solubility

-

Application

  • A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole Blood.: BIS-TRIS propane is used as a buffering agent in the capillary electrophoresis method to assess global DNA methylation levels in human whole blood. The buffer′s role is crucial in maintaining the stability and reproducibility of the electrophoretic conditions (Zinellu et al., 2019).

  • Low potential manganese ions as efficient electron donors in native anoxygenic bacteria.: BIS-TRIS propane is used to buffer the experimental conditions in studying the role of low potential manganese ions as electron donors in bacteria. The findings enhance our understanding of electron transfer processes in microbial systems (Deshmukh et al., 2018).

  • Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection.: This research uses BIS-TRIS propane in a capillary electrophoresis method to evaluate DNA methylation levels. The buffer′s properties ensure accurate and reliable detection, which is crucial for epigenetic studies (Zinellu et al., 2017).

Other Notes

Easily compare specifications for BIS-TRIS propane products with the BIS-TRIS propane specification table.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Hamed Tahmasbi et al.
Journal of pharmaceutical and biomedical analysis, 171, 1-7 (2019-04-07)
Secreted recombinant activated clotting factor VII activated (rFVIIa) in cell culture media missing gamma-carboxyglutamic acid (Gla) domain as a result of failure in gamma-carboxylation or cell lysis is called Gla-domainless impurity which has less negative charge compared to native rFVIIa.
E Csuhai et al.
Biochemistry, 34(38), 12411-12419 (1995-09-26)
A secreted dibasic cleaving peptidase capable of converting dynorphins into Leu-enkephalin-Arg6 was purified from the medium of EL-4 mouse thymoma cells. The enzyme is a novel metalloendopeptidase with a neutral pH optimum (6.9) and a molecular weight of approximately 130
R Tóth-Boconádi et al.
Biophysical journal, 78(6), 3170-3177 (2000-05-29)
Buffers change the electric signals of light-excited bacteriorhodopsin molecules in purple membrane if their concentration and the pH of the low-salt solution are properly selected. "Positive" buffers produce a positive component, and "negative" buffers a negative component in addition to
R Coronado et al.
The Journal of general physiology, 79(4), 529-547 (1982-04-01)
A collection of organic cations has been used to probe the gross structural features of the ionic diffusion pathway in a K+-selective channel from sarcoplasmic reticulum (SR). Channels were incorporated into planar phospholipid bilayer membranes, and single-channel currents were measured
S J Smith et al.
The Journal of biological chemistry, 259(15), 9365-9368 (1984-08-10)
The kinetics of binding and hydrolysis of ATP by bovine cardiac myosin subfragment 1 has been reinvestigated. More than 90% of the total fluorescence amplitude associated with ATP hydrolysis occurs with an apparent second-order rate constant of 8.1 X 10(5)

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