47671

Formamide

BioUltra, Molecular Biology, ≥99.5% (T)

• Synonym(s): Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• UNSPSC Code: 12352111
• NACRES: NA.25
• PubChem Substance ID: 57651075
• EC Number: 200-842-0
• Beilstein/REAXYS Number: 505995
• MDL number: MFCD00007941
• Grade: Molecular Biology
• Assay: ≥99.5% (T)
• Technique(s): FISH: suitable; hybridization: suitable; protein extraction: suitable
• Application(s): genomic analysis
• Bp: 210 °C (lit.)
• Vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)

Properties

• Product Name: Formamide, BioUltra, Molecular Biology, ≥99.5% (T)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• SMILES string: NC=O
• grade: Molecular Biology
• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• product line: BioUltra
• assay: ≥99.5% (T)
• form: liquid
• autoignition temp.: 932 °F
• technique(s): FISH: suitable; hybridization: suitable; protein extraction: suitable
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• ign. residue: ≤0.1%
• refractive index: n20/D 1.447 (lit.); n20/D 1.447
• pH: 7-9 (25 °C, 1 M in H₂O)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• solubility: H₂O: 10 M at 20 °C, clear, colorless
• density: 1.134 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤1 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• cation traces: Ag: ≤5 mg/kg; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; NH₄⁺: ≤1000 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: ≤1 mg/kg
• λ: 10 M in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.4; λ: 280 nm A_max: ≤0.1
• application(s): genomic analysis
• Quality Level: 200

Description

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Formamide has been used:

• as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
• in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
• in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
• to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
• as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent

Biochem/physiol Actions

Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.

Features and Benefits

• Versatile and adaptable for a wide variety of laboratory and research applications
• BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

General description

Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

For the preparation of formamide denaturing polyacrylamide gels[1]; In situ hybridization on human chromosomes[2]

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H351,H360D,H373
• pcodes: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• target_organs: Blood
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 1
• flash_point_f: 305.6 °F
• flash_point_c: 152 °C
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter