SRP5270

SIRT1, active, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): SIR2L1
• CAS Number: 1391438-73-5
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 1573-2415 RLU/min·ng
• mol wt: ~84 kDa
• NCBI accession no.: NM_012238
• application(s): genomic analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SIRT1(23411)

Description

General description

Sirtuin 1 (SIRT1) is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor. SIRT1 is also called as NAD-dependent deacetylase sirtuin-1. This gene is located on human chromosome 10q21.3.[1]

Biochem/physiol Actions

Sirtuin 1 (SIRT1) plays an important role in neuronal proliferation and vascular cells.[1] It also plays an athero-protective role. Mutations in the SIRT1 gene affects the metabolism, cholesterol and fat deposition.[2] SIRT1 has the ability to intermediate the insulin resistance through an inflammatory process, reactive oxygen species, gluconeogenesis and adiponectin level.[1] It is an NAD(+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein. Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53 indicating that SIRT1 is involved in the regulation of p53 function via deacetylation.

Physical form

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H360D
• Precautionary Statements: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable