05-321MG

Anti-Phosphotyrosine Antibody, clone 4G10^®

Name: Anti-Phosphotyrosine Antibody, clone 4G10<SUP>®</SUP>
Brand: MM

clone 4G10®, Upstate^®, from mouse

Sinónimos:

Clone 4G10 Anti-Phosphotyrosine, Phosphotyrosine Detection Antibody

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Código UNSPSC:

12352203

eCl@ss:

32160702

NACRES:

NA.41

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origen biológico

mouse

Nivel de calidad

100

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

4G10®, monoclonal

reactividad de especies (predicha por homología)

all

fabricante / nombre comercial

Upstate^®

técnicas

immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG2bκ

Condiciones de envío

wet ice

modificación del objetivo postraduccional

phosphorylation (pTyr)

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Este artículo	16-103	16-204	16-104
Sigma-Aldrich 05-321MG Anti-Phosphotyrosine Antibody, clone 4G10^®	Sigma-Aldrich 16-103 Anti-Phosphotyrosine Antibody, clone 4G10^®, Biotin Conjugate	Sigma-Aldrich 16-204 Anti-Phosphotyrosine Antibody, recombinant clone 4G10^®, biotin conjugate	Sigma-Aldrich 16-104 Anti-Phosphotyrosine Antibody, clone 4G10^®, FITC conjugate
antibody form purified from hybridoma cell culture	antibody form purified antibody	antibody form purified antibody	antibody form -
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone 4G10®, monoclonal	clone 4G10®, monoclonal	clone 4G10®, monoclonal	clone 4G10®, monoclonal
isotype IgG2bκ	isotype IgG2bκ	isotype IgG2bκ	isotype -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
technique(s) immunoprecipitation (IP): suitable, western blot: suitable	technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) western blot: suitable	technique(s) immunocytochemistry: suitable

Descripción general

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Aplicación

Anti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.

Forma física

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10 (FOX-NY [NS-1 derivative] myeloma x spleen cells).

Nota de análisis

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Routinely evaluated on EGF-treated human A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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ZMS16282

Anti-phosphotyrosine Antibody, clone 4G10 ZooMAb^® Mouse Monoclonal, recombinant, expressed in HEK 293 cells

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Anti-Phosphotyrosine Antibody, clone 4G10®

clone 4G10®, Upstate®, from mouse