07-1562

Anti-EZH2 Antibody

serum, Upstate^®

• Sinónimos: SETDB1, EC 2.1.1.43 ; ; SET domain bifurcated 1 ; ; ERG-associated protein with SET domain ; ; ESET ; ; Histone H3-K9 methyltransferase 4 ; ; H3-K9-HMTase 4 ; ; Lysine N-methyltransferase 1E
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• conjugado: unconjugated
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: rat, mouse, human
• fabricante / nombre comercial: Upstate^®
• técnicas: western blot: suitable
• Nº de acceso GenBank: BC010858
• Nº de acceso NCBI: NM_004456.3; NM_152998.1
• Nº de acceso UniProt: Q15910
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... EZH2(2146)

Descripción

Descripción general

95-100kDa

Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro.. EZH2, also known as ENX-1, is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are co expressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.

Inmunógeno

KLH-conjugated, His-tagged fusion protein corresponding to residues 406-746 of human EHZ2 (similar to enhancer of zeste (Drosophila) homolog 2). Based on accession # BC010858.

Aplicación

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Histones

Use Anti-EZH2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EZH2 also known as SET domain bifurcated 1, ERG-associated protein with SET domain.

Acciones bioquímicas o fisiológicas

Recognizes EZH2, Mr ~100 kDa

Forma física

Serum

Nota de preparación

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
A431 cell lysate

Routinely evaluated by immunoblot analysis

Otras notas

Replaces: 04-1047

Información legal

GenBank is a registered trademark of United States Department of Health and Human Services

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2