12-500
HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer
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Acerca de este artículo
UNSPSC Code:
41105330
NACRES:
NA.42
eCl@ss:
32011807
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Servicio técnico
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Permítanos ayudarlebiological source
human
form
liquid
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
shipped in
dry ice
Quality Level
1 of 4
Este artículo | RAB0157 | RAB0152 | RAB0151 |
|---|---|---|---|
| technique(s) western blot: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
| form liquid | form - | form - | form - |
| biological source human | biological source - | biological source - | biological source - |
| manufacturer/tradename Upstate® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Biochem/physiol Actions
HeLa
General description
EGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)
Physical form
100 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.
Preparation Note
Stable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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