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Nombre del producto
Chromolith® NH2, L × I.D. 100 mm x 4.6 mm HPLC column
Materiales
PEEK column
Nivel de calidad
Agency
suitable for USP L8
Línea del producto
Chromolith®
Características
endcapped: no
envase
1 ea of
Extensión del etiquetado
5% Carbon loading
Parámetros
200 bar pressure
45 °C max. temp.
técnicas
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)
columna L × D.I.
10 cm × 4.6 mm
Matriz
monolithic silica
grupo activo de la matriz
NH2 bonding phase
tamaño de poro
13 nm mesoporosity
2 μm macroporosity
Intervalo de pH operativo
2-7.5
técnica de separación
hydrophilic interaction (HILIC)
normal phase
reversed phase
temp. de almacenamiento
no temp limit
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Categorías relacionadas
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Este artículo | 1.52026 | 1.52027 | 1.52048 |
|---|---|---|---|
| separation technique hydrophilic interaction (HILIC), reversed phase, normal phase | separation technique hydrophilic interaction (HILIC), normal phase, reversed phase | separation technique hydrophilic interaction (HILIC), normal phase, reversed phase | separation technique hydrophilic interaction (HILIC), normal phase, reversed phase |
| matrix active group NH2 bonding phase | matrix active group NH2 bonding phase | matrix active group NH2 bonding phase | matrix active group Cyano (CN) bonding phase |
| technique(s) HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium), LC/MS: suitable | technique(s) HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (Low - Medium) | technique(s) HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (Low - Medium) | technique(s) HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High) |
| product line Chromolith® | product line Chromolith® | product line Chromolith® | product line Chromolith® |
| matrix monolithic silica | matrix monolithic silica | matrix monolithic silica | matrix monolithic silica |
| pore size 13 nm mesoporosity, 2 μm macroporosity | pore size 13 nm mesoporosity, 2 μm macroporosity | pore size 13 nm mesoporosity, 2 μm macroporosity | pore size 13 nm mesoporosity, 2 μm macroporosity |
Descripción general
Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030 with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52030]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Nota de análisis
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.3
Información legal
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
A new HPLC-RI method is proposed for the analysis of Lactose in milk using a Chromolith® NH2 column.
This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.
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