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Merck

1.52028

Chromolith® NH2 (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

Sinónimos:

Chromolith® Performance NH2 100-4.6 HPLC column

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Código UNSPSC:
41115700
NACRES:
SB.52

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Nombre del producto

Chromolith® NH2, L × I.D. 100 mm x 4.6 mm HPLC column

Materiales

PEEK column

Nivel de calidad

Agency

suitable for USP L8

Línea del producto

Chromolith®

Características

endcapped: no

envase

1 ea of

Extensión del etiquetado

5% Carbon loading

Parámetros

200 bar pressure
45 °C max. temp.

técnicas

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)

columna L × D.I.

10 cm × 4.6 mm

Matriz

monolithic silica

grupo activo de la matriz

NH2 bonding phase

tamaño de poro

13 nm mesoporosity
2 μm macroporosity

Intervalo de pH operativo

2-7.5

técnica de separación

hydrophilic interaction (HILIC)
normal phase
reversed phase

temp. de almacenamiento

no temp limit

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1 of 4

Este artículo
1.520261.520271.52048
separation technique

hydrophilic interaction (HILIC), reversed phase, normal phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

separation technique

hydrophilic interaction (HILIC), normal phase, reversed phase

matrix active group

NH2 bonding phase

matrix active group

NH2 bonding phase

matrix active group

NH2 bonding phase

matrix active group

Cyano (CN) bonding phase

technique(s)

HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium), LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (Low - Medium)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (Low - Medium)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

pore size

13 nm mesoporosity, 2 μm macroporosity

Descripción general

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030 with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52030]

The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Nota de análisis

Pressure: ≤ 15 bar
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.3

Información legal

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

A new HPLC-RI method is proposed for the analysis of Lactose in milk using a Chromolith® NH2 column.

This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.

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