16-282

Anti-Phosphotyrosine Antibody, clone 4G10^®, Magnetic Bead Conjugate

clone 4G10®, from mouse

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 4G10®, monoclonal
• reactividad de especies (predicha por homología): all
• técnicas: immunoprecipitation (IP): suitable
• isotipo: IgG2bκ
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pTyr)
• Información sobre el gen: human ... PID1(55022)

Descripción

Descripción general

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

Inmunógeno

Phosphotyramine-KLH

Aplicación

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate validated for use in IP.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Acciones bioquímicas o fisiológicas

This antibody recognizes tyrosine-phosphorylated proteins from all species

Forma física

20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.

Format: Purified

Protein G-Sepharose Chromatography

Nota de preparación

Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE

Nota de análisis

100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).

Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

Información legal

4G10 is a registered trademark of Upstate Group, Inc.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2