Merck
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407707

Sigma-Aldrich

PhosphoDetect Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb

liquid, Calbiochem®

origen biológico

rabbit

Nivel de calidad

100

antibody product type

primary antibodies

clon

polyclonal

formulario

liquid

contiene

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

isotipo

IgG

enviado en

wet ice

temp. de almacenamiento

−70°C

Gene Information

human ... INSR(3643)

Descripción general

Note: 1 T = 1 test. Sufficient for 10 Western mini blots.
Recognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr1162 and Tyr1163 in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr1135 and Tyr1136.
This PhosphoDetect Anti-Insulin Receptor (pTyr1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr1162/1163).
Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr1162/1163 and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr1162/1163.

Inmunógeno

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1162/1163 phosphorylation sites of human insulin receptor

Envase

10 tests in Plastic ampoule

Aplicación

ELISA (see comments)

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

Advertencia

Toxicity: Standard Handling (A)

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Otras notas

Bevan, P. 2001. J. Cell Sci.114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest.103, 931.
Schmid, E., at al. 1998. FASEB J.12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ebina, Y., et al. 1985. Cell40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation27, 83.
Grunberger, G., et al. 1984. Science223, 932.
The immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

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Certificate of Quality

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