5.30650

Luciferase Substrate, CycLuc1

• Sinónimos: Luciferase Substrate, CycLuc1, 2-(6,7-dihydro-5h-thiazolo[4,5-f]indol-2-yl)-4,5-dihydrothiazole-4-carboxylicacid
• Fórmula empírica (notación de Hill): C₁₃H₁₁N₃O₂S₂
• Peso molecular: 305.38
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Ensayo: ≥94% (HPLC)
• Nivel de calidad: 100
• Formulario: powder
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: yellow
• solubilidad: DMSO: 100 mg/mL
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

A synthetic cyclic alkylamino luciferin that exhibitsproperties that are superior to those of D-luciferin. Binds to luciferase with higheraffinity and emits about 5.7-fold more light than aminoluciferin and3.2-fold more light than D-luciferin. Its superior light output is maintainedover a wide range (1-100 µM). Shown to readily cross the blood-brain barrier inmice and provides much improved noninvasive bioluminescence imaging (BLI) ofthe brain at a lower dose when compared to D-luciferin. Yields about 10-foldhigher bioluminescent signal than D-luciferin when Luc2 luciferase expressing4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice.A rapid (4-5 min), long-lasting, and steady signal peak is observed followingan i.v injection of CycLuc1 in mice.

Aplicación

Luciferase Substrate, CycLuc1 has been used for transfecting HEK293 cells to study the inhibition of intracellular luciferase crystallization by D-luciferin and its synthetic analogues.[1]

Acciones bioquímicas o fisiológicas

Cell permeable: yes

Primary Target
Luciferase

Envase

Packaged under inert gas

Otras notas

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Toxicity: Standard Handling (A)

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable