AB10181

Anti-Pumilio-2 (PUM2) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: pumilio homolog 2 (Drosophila), pumilio homolog 2, pumilio (Drosphila) homolog 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rhesus macaque, mouse, human
• reactividad de especies (predicha por homología): chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), canine (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_056132
• Nº de acceso UniProt: Q8TB72
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PUM2(23369)

Descripción

Descripción general

PUM2 is a member of the Puf family of RNA-binding proteins, which are believed to play a role in the regulation of posttranscriptional gene expression. Members of the Puf family of RNA binding proteins function as repressors of translation when bound to the 3-untranslated region of RNA transcripts.

~120 kDa observed.
Non specific band may be observed at 48 kDa

Inmunógeno

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human Pumilio-2.

Aplicación

Detect Pumilio-2 (PUM2) using this Anti-Pumilio-2 (PUM2) Antibody validated for use in IC, IH & WB.

Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected Pumilio-2 in human testis and teratoma tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-2 in human and mouse embryonic stem cells.

Research Category
Stem Cell Research

Research Sub Category
RNA Metabolism & Binding Proteins

Acciones bioquímicas o fisiológicas

This antibody recognizes Pumilio-2 near the N-terminus.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Nota de preparación

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Mouse embryonic stem cell lysate

Evaluated by Western Blot on mouse embryonic stem cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Pumilio-2 (PUM2) in 10 µg of mouse embryonic stem cell lysate.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable