AB5583
Anti-Nerve Growth Factor Antibody, pro
Chemicon®, from rabbit
Sinónimos:
NGF, pro
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Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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rabbit
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
affinity purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
polyclonal
purificado por
affinity chromatography
reactividad de especies
rat
fabricante / nombre comercial
Chemicon®
técnicas
immunohistochemistry: suitable
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... NTF3(4908)
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Este artículo | AB9040 | MAB5260 | AB1532P |
|---|---|---|---|
| biological source rabbit | biological source rabbit | biological source mouse | biological source rabbit |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| antibody form affinity purified immunoglobulin | antibody form serum | antibody form purified immunoglobulin | antibody form affinity purified immunoglobulin |
| technique(s) immunohistochemistry: suitable, western blot: suitable | technique(s) immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) ELISA: suitable | technique(s) immunohistochemistry: suitable, western blot: suitable |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| clone polyclonal | clone polyclonal | clone 27/21, monoclonal | clone polyclonal |
Inmunógeno
Synthetic corresponding to amino acids 84-104 of rat proNGF (Accession P25427).
Aplicación
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
This Anti-Nerve Growth Factor Antibody, pro is validated for use in WB, IH for the detection of Nerve Growth Factor.
Western blotting: 1:200-1:500 using ECL on His-fusion protein containing the entire prodomain of rat proNGF.
Immunohistochemistry on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Acciones bioquímicas o fisiológicas
Recognizes proNGF (Nerve Growth Factor).
SPECIES REACTIVITIES:
The immunogen sequence has 100% homology with mouse and 76% homology with human proNGF. Reactivity with other species has not yet been tested.
SPECIES REACTIVITIES:
The immunogen sequence has 100% homology with mouse and 76% homology with human proNGF. Reactivity with other species has not yet been tested.
Forma física
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.025% sodium azide. Reconstitute with 50 mL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Nota de preparación
Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Nota de análisis
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Modulation of rat parasympathetic cardiac ganglion phenotype and NGF synthesis by adrenergic nerves.
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Autonomic neuroscience : basic & clinical, 145(1-2), 17-26 (2008-11-21)
Cardiac function is regulated by interactions among intrinsic and extrinsic autonomic neurons, and the mechanisms responsible for organizing these circuits are poorly understood. Parasympathetic neurons elsewhere synthesize the neurotrophin NGF, which may promote postganglionic axonal associations where parasympathetic axons inhibit
María Eugenia Bogetti et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 71, 111-121 (2018-08-31)
A decrease in the concentration of oxygen in the blood and tissues (hypoxia) produces important, sometimes irreversible, damages in the central nervous system (CNS) both during development and also postnatally. The present work aims at analyzing the expression of nerve
F Jardí et al.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 24(12), e580-e590 (2012-10-18)
Nerve growth factor (NGF)-mucosal mast cell (MMC) interaction has been implicated in the remodeling of enteric circuitries and associated functional changes. We investigated the involvement of NGF and its receptor TrkA in the altered colonic contractile activity observed in the
Zheng-Mao Li et al.
MedComm, 3(3), e128-e128 (2022-07-01)
Accumulating evidence indicates that epilepsy has a higher risk of inducing memory impairment and dementia. However, the underlying onset mechanism remains unclear. Here, we found that mice with spontaneous epilepsy induced by endothelial CDK5 deficiency exhibited hippocampal-dependent memory impairment at
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